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Calponin 2 antibody, Internal

Anti-Calponin 2 antibody, Internal used in IHC (Paraffin sections) (IHC-P). GTX88377
Anti-Calponin 2 antibody, Internal used in Western Blot (WB). GTX88377

Cat. No. GTX88377

Host

 Goat

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μg ($399)
See all Calponin 2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.03-0.1μg/ml
IHC-P 2.5μg/ml
Not tested in other applications.

Calculated MW

34 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX88377-PEP).

Product Note

This antibody is expected to recognize both reported isoforms (NP_004359.1; NP_958434.1), but it is not expected to cross-react with CCN3.

PROPERTIES

Form

Liquid

Buffer

TBS, 0.5% BSA

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence C-DPGEVPEYPPYYQEE, from the internal region (near C Terminus) of the protein sequence according to NP_004359.1; NP_958434.1.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

calponin 2

Background

The protein encoded by this gene, which can bind actin, calmodulin, troponin C, and tropomyosin, may function in the structural organization of actin filaments. The encoded protein could play a role in smooth muscle contraction and cell adhesion. Several pseudogenes of this gene have been identified, and are present on chromosomes 1, 2, 3, 6, 9, 11, 13, 15, 16, 21 and 22. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jan 2015]

Database

Research Area

DATA IMAGES

Anti-Calponin 2 antibody, Internal used in IHC (Paraffin sections) (IHC-P). GTX88377

GTX88377 IHC-P Image

IHC-P analysis of human placenta using GTX88377 Calponin 2 antibody, Internal.
Antigen retrieval : citrate buffer pH 6
Dilution : 2.5μg/ml

Anti-Calponin 2 antibody, Internal used in Western Blot (WB). GTX88377

GTX88377 WB Image

WB analysis of HepG2 lysates using GTX88377 Calponin 2 antibody, Internal.
Dilution : 0.03μg/ml
Loading : 35μg protein in RIPA buffer

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399