APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
HeLa (200 μM CoCl2 treatment for 24 hr) , 30u g HeLa (200 μM CoCl2 treatment for 48 hr) , HeLa (1% O2 treatment for 24 hr) , HeLa (1% O2 treatment for 48 hr)
Product Note
This antibody was raised against human Carbonic Anhydrase IX Extracellular domain.
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the Extracellular domain of human Carbonic Anhydrase IX. The exact sequence is proprietary.
Purification
Affinity purified by Protein G.
Conjugation
Unconjugated
RRID
AB_2888148
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
carbonic anhydrase 9 , CAIX , MN
Cellular Localization
Nucleus , nucleolus , Cell membrane , Cell projection , microvillus membrane
Background
Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. CA IX is a transmembrane protein and the only tumor-associated carbonic anhydrase isoenzyme known. It is expressed in all clear-cell renal cell carcinoma, but is not detected in normal kidney or most other normal tissues. It may be involved in cell proliferation and transformation. This gene was mapped to 17q21.2 by fluorescence in situ hybridization, however, radiation hybrid mapping localized it to 9p13-p12. [provided by RefSeq]
Database
Research Area
DATA IMAGES
![Anti-Carbonic Anhydrase IX antibody [GT14212] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629747](/templates/default/js/lightbox/images/loading.gif "Anti-Carbonic Anhydrase IX antibody [GT14212] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629747")
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GTX629747 ICC/IF Image
Carbonic Anhydrase IX antibody [GT14212] detects Carbonic Anhydrase IX protein at membrane by immunofluorescent analysis.
Sample: 200μM CoCl2 for 48 hr A431 cells were fixed in ice-cold MeOH for 5 min.
Green: Carbonic Anhydrase IX protein stained by Carbonic Anhydrase IX antibody [GT14212] (GTX629747) diluted at 1:1000.
Blue: Hoechst 33342 staining.
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GTX629747 WB Image
Carbonic Anhydrase IX antibody [GT14212] detects Carbonic Anhydrase IX protein by Western blot analysis.
A. 30 μg HeLa whole cell lysate/extract (untreated)
B. 30 μg HeLa whole cell lysate/extract (200 μM CoCl2 treatment for 24 hr)
C. 30u g HeLa whole cell lysate/extract (200 μM CoCl2 treatment for 48 hr)
D. 30 μg HeLa whole cell lysate/extract (untreated)
E. 30 μg HeLa whole cell lysate/extract (1% O2 treatment for 24 hr)
F. 30 μg HeLa whole cell lysate/extract (1% O2 treatment for 48 hr)
7.5 % SDS-PAGE
Carbonic Anhydrase IX antibody [GT14212] (GTX629747) dilution: 1:1000
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REFERENCE
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REVIEW
There are currently no reviews for Carbonic Anhydrase IX antibody [GT14212] (GTX629747). Be the first to share your experience with this product.
![Anti-Carbonic Anhydrase IX antibody [GT14212] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629747](/upload/website/prouct_img/small/GTX629747/GTX629747_41428_IFA_w_23061202_845.webp "Anti-Carbonic Anhydrase IX antibody [GT14212] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629747")
![Anti-Carbonic Anhydrase IX antibody [GT14212] used in Western Blot (WB). GTX629747](/upload/website/prouct_img/small/GTX629747/GTX629747_41428_WB_Hypoxia_w_23061202_596.webp "Anti-Carbonic Anhydrase IX antibody [GT14212] used in Western Blot (WB). GTX629747")
![Anti-Carbonic Anhydrase IX antibody [GT14212] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629747](/upload/website/prouct_img/normal/GTX629747/GTX629747_41428_IFA_w_23061202_845.webp "Anti-Carbonic Anhydrase IX antibody [GT14212] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629747")
![Anti-Carbonic Anhydrase IX antibody [GT14212] used in Western Blot (WB). GTX629747](/upload/website/prouct_img/normal/GTX629747/GTX629747_41428_WB_Hypoxia_w_23061202_596.webp "Anti-Carbonic Anhydrase IX antibody [GT14212] used in Western Blot (WB). GTX629747")