GeneTex

  • Country / Location Selection

United States (US)

Citrate synthase antibody [GT2061]

Anti-Citrate synthase antibody [GT2061] used in Western Blot (WB). GTX628144
Anti-Citrate synthase antibody [GT2061] used in Western Blot (WB). GTX628144
Anti-Citrate synthase antibody [GT2061] used in Western Blot (WB). GTX628144
Anti-Citrate synthase antibody [GT2061] used in IHC (Paraffin sections) (IHC-P). GTX628144
Anti-Citrate synthase antibody [GT2061] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX628144
Anti-Citrate synthase antibody [GT2061] used in Western Blot (WB). GTX628144
Anti-Citrate synthase antibody [GT2061] used in Western Blot (WB). GTX628144

Cat. No. GTX628144

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 GT2061

Isotype

 IgG2b

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat, Zebrafish
Package
100 μl ($399),
25 μl ($169)
See all Citrate synthase products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

52 kDa. ( Note )

Positive Control

zebrafish heart , 293T , A431 , HepG2 , mouse brain , zebrafish brain , rat brain

Predict Reactivity

Bovine, Pig, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Citrate synthetase. The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2888021

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

citrate synthase

Cellular Localization

Mitochondrion matrix

Background

The protein encoded by this gene is a Krebs tricarboxylic acid cycle enzyme that catalyzes the synthesis of citrate from oxaloacetate and acetyl coenzyme A. The enzyme is found in nearly all cells capable of oxidative metablism. This protein is nuclear encoded and transported into the mitochondrial matrix, where the mature form is found. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Citrate synthase antibody [GT2061] used in Western Blot (WB). GTX628144

GTX628144 WB Image

Rat tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Citrate synthetase antibody [GT2061] (GTX628144) diluted at 1:1000.

Anti-Citrate synthase antibody [GT2061] used in Western Blot (WB). GTX628144

GTX628144 WB Image

Citrate synthetase antibody [GT2061] detects CS protein by Western blot analysis.
A. 30 μg zebrafish heart lysate/extract
10 % SDS-PAGE
Citrate synthetase antibody [GT2061] (GTX628144) dilution: 1:1000

Anti-Citrate synthase antibody [GT2061] used in Western Blot (WB). GTX628144

GTX628144 WB Image

Citrate synthetase antibody [GT2061] detects CS protein by Western blot analysis.
A. 30 μg zebrafish brain lysate/extract
10 % SDS-PAGE
Citrate synthetase antibody [GT2061] (GTX628144) dilution: 1:1000

Anti-Citrate synthase antibody [GT2061] used in IHC (Paraffin sections) (IHC-P). GTX628144

GTX628144 IHC-P Image

Citrate synthetase antibody [GT2061] detects CS protein at cytosol on hepatoma by immunohistochemical analysis.
Sample: Paraffin-embedded hepatoma.
Citrate synthetase antibody [GT2061] (GTX628144) dilution: 1:200.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Citrate synthase antibody [GT2061] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX628144

GTX628144 ICC/IF Image

Citrate synthetase antibody [GT2061] detects Citrate synthetase protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: Citrate synthetase protein stained by Citrate synthetase antibody [GT2061] (GTX628144) diluted at 1:500.
Blue: Hoechst 33342 staining.
Anti-Citrate synthase antibody [GT2061] used in Western Blot (WB). GTX628144

GTX628144 WB Image

Citrate synthetase antibody [GT2061] detects CS protein by Western blot analysis.
A. 50 μg mouse brain lysate/extract
12 % SDS-PAGE
Citrate synthetase antibody [GT2061] (GTX628144) dilution: 1:1000

Anti-Citrate synthase antibody [GT2061] used in Western Blot (WB). GTX628144

GTX628144 WB Image

Citrate synthetase antibody detects CS protein by Western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg A431 whole cell lysate/extract
C. 30 μg HepG2 whole cell lysate/extract
10 % SDS-PAGE
Citrate synthetase antibody (GTX628144) dilution: 1:1000

REFERENCE

There are currently no references for Citrate synthase antibody [GT2061] (GTX628144). Be the first to share your publications with this product.
Submit a Reference

REVIEW

There are currently no reviews for Citrate synthase antibody [GT2061] (GTX628144). Be the first to share your experience with this product.
Submit a Review
SDS
PBS.pdf
Package List Price ($)
$ 399
$ 169
Custom Conjugations