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DARPP-32 (phospho Thr34) antibody

Anti-DARPP-32 (phospho Thr34) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX55025
Anti-DARPP-32 (phospho Thr34) antibody used in Western Blot (WB). GTX55025
Anti-DARPP-32 (phospho Thr34) antibody used in IHC (Paraffin sections) (IHC-P). GTX55025

Cat. No. GTX55025

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all DARPP-32 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:1000
ICC/IF 1:100 - 1:500
IHC-P 1:100 - 1:200
Not tested in other applications.

Calculated MW

23 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

0.42% Potassium Phosphate, 0.87% NaCl, 30% Glycerol

Preservative

0.01% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of DARPP-32. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

protein phosphatase 1 regulatory inhibitor subunit 1B , DARPP-32 , DARPP32

Cellular Localization

Cytoplasm

Background

This gene encodes a bifunctional signal transduction molecule. Dopaminergic and glutamatergic receptor stimulation regulates its phosphorylation and function as a kinase or phosphatase inhibitor. As a target for dopamine, this gene may serve as a therapeutic target for neurologic and psychiatric disorders. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]

Database

Research Area

DATA IMAGES

Anti-DARPP-32 (phospho Thr34) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX55025

GTX55025 ICC/IF Image

ICC/IF analysis of formalin-fixed HepG2 cells using GTX55025 DARPP-32 (phospho Thr34) antibody.
Red : Primary antibody
Blue : DAPI
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minutes

Anti-DARPP-32 (phospho Thr34) antibody used in Western Blot (WB). GTX55025

GTX55025 WB Image

WB analysis of human brain (A), NIH3T3 (B), rat muscle (C) whole cell lysates using GTX55025 DARPP-32 (phospho Thr34) antibody.

Anti-DARPP-32 (phospho Thr34) antibody used in IHC (Paraffin sections) (IHC-P). GTX55025

GTX55025 IHC-P Image

IHC-P analysis of formalin fixed human breast cancer tissue section using GTX55025 DARPP-32 (phospho Thr34) antibody.
Antigen retrieval : Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0)

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399