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DDDDK tag antibody [M2-RB]

Recombinant antibody Comparison
Anti-DDDDK tag antibody [M2-RB] used in Western Blot (WB). GTX640415
Anti-DDDDK tag antibody [M2-RB] used in ELISA (ELISA). GTX640415
Anti-DDDDK tag antibody [M2-RB] used in Western Blot (WB). GTX640415
Anti-DDDDK tag antibody [M2-RB] used in ELISA (ELISA). GTX640415
Anti-DDDDK tag antibody [M2-RB] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX640415

Cat. No. GTX640415

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 M2-RB

Isotype

 IgG

Application

 WB, ICC/IF, ELISA

Reactivity

 Species independent, Human
Package
100 μl ($399),
25 μl ($169)
See all DDDDK tag products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF Assay dependent
ELISA Assay dependent
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

batch dependent (Please refer to the vial label for the specific concentration.)

Immunogen

The immunogen used to generate this antibody corresponds to DDDDK tag.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Research Area

DATA IMAGES

Anti-DDDDK tag antibody [M2-RB] used in Western Blot (WB). GTX640415

GTX640415 WB Image

Comparison

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membranes were blotted with DDDDK tag antibody [M2-RB] (GTX640415) diluted at 1:10000 and competitor's antibody (Highly Cited Antibody) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-DDDDK tag antibody [M2-RB] used in ELISA (ELISA). GTX640415

GTX640415 ELISA Image

Indirect ELISA analysis was performed by coating the plate with recombinant Multiple Tags Positive Control ( 6xHis-MBP-DDDDK-V5-HSV-HA-T7-Myc-S) (GTX130342-pro) (57.14-0.89 nM). Coated protein was probed with DDDDK tag antibody [HL3005] (GTX640415) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect the bound primary antibody.

Anti-DDDDK tag antibody [M2-RB] used in Western Blot (WB). GTX640415

GTX640415 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with DDDDK tag antibody [M2-RB] (GTX640415) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-DDDDK tag antibody [M2-RB] used in ELISA (ELISA). GTX640415

GTX640415 ELISA Image

Indirect ELISA analysis was performed by coating the plate with recombinant Multiple Tags Positive Control (GST-DDDDK-V5-HSV-HA-T7-Myc-S) (GTX130343-pro) (72.73-1.14 nM). Coated protein was probed with DDDDK tag antibody [HL3005] (GTX640415) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect the bound primary antibody.

Anti-DDDDK tag antibody [M2-RB] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX640415

GTX640415 ICC/IF Image

DDDDK tag antibody [M2-RB] detects DDDDK tag protein by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in ice-cold MeOH for 5 min.
Green: DDDDK tag stained by DDDDK tag antibody [M2-RB] (GTX640415) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations