APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:1000-1:10000 |
Assay dependent |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the C-terminus region of human DDX4. The exact sequence is proprietary.
Purification
Affinity purified by Protein A.
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
DEAD-box helicase 4 , VASA
Cellular Localization
Cytoplasm , Cytoplasm, perinuclear region
Background
DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which is a homolog of VASA proteins in Drosophila and several other species. The gene is specifically expressed in the germ cell lineage in both sexes and functions in germ cell development. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2009]
Database
Research Area
DATA IMAGES
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GTX636961 IHC-P Image
DDX4 antibody [HL1487] detects DDX4 protein by immunohistochemical analysis.Sample: Paraffin-embedded rat testis.DDX4 stained by DDX4 antibody [HL1487] (GTX636961) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX636961 IHC-P Image
DDX4 antibody [HL1487] detects DDX4 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse testis. DDX4 stained by DDX4 antibody [HL1487] (GTX636961) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX636961 WB Image
Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with DDX4 antibody [HL1487] (GTX636961) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX636961 WB Image
Rat tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with DDX4 antibody [HL1487] (GTX636961) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX636961 WB Image
Human tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with DDX4 antibody [HL1487] (GTX636961) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
|
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GTX636961 IHC-P Image
DDX4 antibody [HL1487] detects DDX4 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded rat testis.DDX4 stained by DDX4 antibody [HL1487] (GTX636961) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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REFERENCE
There are currently no references for DDX4 antibody [HL1487] (GTX636961). Be the first to share your publications with this product.
REVIEW
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