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Dectin-1 antibody [2A11] (Low endotoxin, azide free)

Anti-Dectin-1 antibody [2A11] (Low endotoxin, azide free) used in IHC (Frozen sections) (IHC-Fr). GTX41471
Anti-Dectin-1 antibody [2A11] (Low endotoxin, azide free) used in IHC (Frozen sections) (IHC-Fr). GTX41471
Anti-Dectin-1 antibody [2A11] (Low endotoxin, azide free) used in IHC (Frozen sections) (IHC-Fr). GTX41471
Anti-Dectin-1 antibody [2A11] (Low endotoxin, azide free) used in IHC (Frozen sections) (IHC-Fr). GTX41471
Anti-Dectin-1 antibody [2A11] (Low endotoxin, azide free) used in Flow cytometry (FACS). GTX41471

Cat. No. GTX41471

Reference 9

Host

 Rat

Clonality

 Monoclonal

Clone Name

 2A11

Isotype

 IgG2b

Application

 IHC-Fr, FACS, IP, Neutralizing/Inhibition

Reactivity

 Mouse
Package
100 μg ($399)
See all Dectin-1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
IHC-Fr Assay dependent
FACS 1/10-1/50
IP Assay dependent
Neutralizing/Inhibition Assay dependent

Note :

FACS
Use 10μl of the suggested working dilution to label 106 cells in 100μl.

Neutralizing/Inhibition
Rat anti Mouse Dectin-1 antibody, clone 2A11 inhibits the binding of zymosan to macrophages via the beta-glucan receptor.
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.

Concentration

1.0 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Dectin-1 transfected NIH3T3 cells and recombinant soluble Dectin-1.

Purification

Protein G purified
From tissue culture supernatant

Endotoxin

< 0.01 EU/μg (determined by the LAL assay)

Conjugation

Unconjugated

RRID

AB_11165950

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

C-type lectin domain family 7, member a , BGR , Clecsf12 , beta-GR

Database

Research Area

DATA IMAGES

Anti-Dectin-1 antibody [2A11] (Low endotoxin, azide free) used in IHC (Frozen sections) (IHC-Fr). GTX41471

GTX41471 IHC-Fr Image

IHC-Fr analysis of mouse lymph node tissue using GTX41471 Dectin-1 antibody [2A11] (Low endotoxin, azide free).

Anti-Dectin-1 antibody [2A11] (Low endotoxin, azide free) used in IHC (Frozen sections) (IHC-Fr). GTX41471

GTX41471 IHC-Fr Image

IHC-Fr analysis of mouse lymph node tissue using GTX41471 Dectin-1 antibody [2A11] (Low endotoxin, azide free).

Anti-Dectin-1 antibody [2A11] (Low endotoxin, azide free) used in IHC (Frozen sections) (IHC-Fr). GTX41471

GTX41471 IHC-Fr Image

IHC-Fr analysis of mouse skin tissue using GTX41471 Dectin-1 antibody [2A11] (Low endotoxin, azide free).

Anti-Dectin-1 antibody [2A11] (Low endotoxin, azide free) used in IHC (Frozen sections) (IHC-Fr). GTX41471

GTX41471 IHC-Fr Image

IHC-Fr analysis of mouse lymph node tissue using GTX41471 Dectin-1 antibody [2A11] (Low endotoxin, azide free).

Anti-Dectin-1 antibody [2A11] (Low endotoxin, azide free) used in Flow cytometry (FACS). GTX41471

GTX41471 FACS Image

FACS analysis of mouse peripheral blood monocytes using GTX41471 Dectin-1 antibody [2A11] (Low endotoxin, azide free).

REFERENCE

Clone Reference

Johnathan Canton et al. Nat Immunol 2021; 22 (2):140-153 The receptor DNGR-1 signals for phagosomal rupture to promote cross-presentation of dead-cell-associated antigens.
Okada Y et al. J Gastroenterol 2021; 56 (9):829-842 Novel probiotic yeast from Miso promotes regulatory dendritic cell IL-10 production and attenuates DSS-induced colitis in mice.
Wang HT et al. J Food Drug Anal 2018; 26 (2):678-687 Characteristics of fucose-containing polysaccharides from submerged fermentation of?Agaricus blazei Murill.
Fujikura D et al. The Journal of Biochemistry 2017; mvx062 Aureobasidium pullulans-cultured fluid induces IL-18 production, leading to Th1-polarization during influenza A virus infection.
Wang HT et al. Journal of Food and Drug Analysis 2017; (Epub) Characteristics of fucose-containing polysaccharides from submerged fermentation of Agaricus blazei Murill.
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REVIEW

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Application Reference
Guo Z et al. J Immunol 2013; 190 (8):4337-48 Antisense oligonucleotide treatment enhances the recovery of acute lung injury through IL-10-secreting M2-like macrophage-induced expansion of CD4+ regulatory T cells.
Clone Reference
Johnathan Canton et al. Nat Immunol 2021; 22 (2):140-153 The receptor DNGR-1 signals for phagosomal rupture to promote cross-presentation of dead-cell-associated antigens.
Okada Y et al. J Gastroenterol 2021; 56 (9):829-842 Novel probiotic yeast from Miso promotes regulatory dendritic cell IL-10 production and attenuates DSS-induced colitis in mice.
Wang HT et al. J Food Drug Anal 2018; 26 (2):678-687 Characteristics of fucose-containing polysaccharides from submerged fermentation of?Agaricus blazei Murill.
Fujikura D et al. The Journal of Biochemistry 2017; mvx062 Aureobasidium pullulans-cultured fluid induces IL-18 production, leading to Th1-polarization during influenza A virus infection.
Wang HT et al. Journal of Food and Drug Analysis 2017; (Epub) Characteristics of fucose-containing polysaccharides from submerged fermentation of Agaricus blazei Murill.
Byun EB et al. Am J Chin Med 2016; 44 (5):1009-26 Immune Enhancing Activity of β-(1,3)-Glucan Isolated from Genus Agrobacterium in Bone-Marrow Derived Macrophages and Mice Splenocytes.
Jackson N et al. Eur J Immunol 2014; 44 (4):962-9 Recognition of Salmonella by Dectin-1 induces presentation of peptide antigen to type B T cells.
Yang LC et al. Evid Based Complement Alternat Med 2013; 2013:458075 a type ii arabinogalactan from anoectochilus formosanus for g-csf production in macrophages and leukopenia improvement in ct26-bearing mice treated with 5-*.
SDS
PBS.pdf
Package List Price ($)
$ 399
Custom Conjugations