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Diablo antibody

Anti-Diablo antibody used in IHC (Paraffin sections) (IHC-P). GTX13817
Anti-Diablo antibody used in Western Blot (WB). GTX13817

Cat. No. GTX13817

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μg ($399)
See all Diablo products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.5 - 2 μg/ml
ICC/IF 1:10 - 1:500
IHC-P 1:10 - 1:500
Not tested in other applications.

Calculated MW

27 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

This rabbit polyclonal antibody was developed against two synthetic peptides corresponding to amino acids 199 to 212 and amino acids 226 to 239 of human Smac (Genbank accession no. AAF87716).

Purification

Protein G purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

diablo IAP-binding mitochondrial protein , DFNA64 , SMAC

Cellular Localization

Mitochondrion

Background

This gene encodes an inhibitor of apoptosis protein (IAP)-binding protein. The encoded mitochondrial protein enters the cytosol when cells undergo apoptosis, and it moderates the caspase inhibition of IAPs. Multiple polyadenylation sites have been found for this gene. Four alternatively spliced transcript variants have been described for this gene, with two of them encoding different isoforms and the other two probably not encoding a protein. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Diablo antibody used in IHC (Paraffin sections) (IHC-P). GTX13817

GTX13817 IHC-P Image

IHC-P analysis of human prostate tissue using GTX13817 Diablo antibody.
Dilution : 10 μg/ml

Anti-Diablo antibody used in Western Blot (WB). GTX13817

GTX13817 WB Image

WB analysis of HeLa cell lysate using GTX13817 Diablo antibody.
Dilution : 2 μg/ml

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399