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United States (US)

Donkey Anti-Mouse IgG antibody, pre-adsorbed (AP)

Cat. No. GTX27062

Reference 1

Host

 Donkey

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, Dot, ELISA, IHC

Reactivity

 Mouse
Package
100 μg ($129)
See all Mouse IgG products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:5000
Dot Assay dependent
ELISA 1:2000-1:20000
IHC 1:500-1:2500
Not tested in other applications.

Product Note

Pre-adsorbed with Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Rabbit, Rat and Sheep serum proteins. May react with immunoglobulins from other species.

PROPERTIES

Form

Liquid

Buffer

50mM Tris-HCl, 150mM NaCl, 1mM MgCl₂, 0.1mM ZnCl₂, 1% BSA, 50% Glycerol

Preservative

0.1% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Mouse IgG whole molecule

Target Isotype

IgG

Purification

Purified by antigen-affinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorptions to remove any unwanted reactivities.
From serum

Conjugation

Alkaline Phosphatase (AP)

RRID

AB_378072

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

REFERENCE

Application Reference

Hadrevi J et al. BMC Musculoskelet Disord 2011; 12 : 181 Protein differences between human trapezius and vastus lateralis muscles determined with a proteomic approach.
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SDS
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 129