APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:500-1:3000 |
Assay dependent |
Not tested in other applications.
Calculated MW
Positive Control
MCF-7 , E2F1-transfected 293T
Product Note
This antibody recognizes E2F-1 that was identified as a cellular protein with DNA binding activity associated with the adenovirus E2 gene promoter.
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No Preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
2.29 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
The region encoding the DP-1 binding site (amino acids 223-332) of E2F-1 expressed in E. coli.
Purification
Protein G purified
From tissue culture supernatant
Conjugation
Unconjugated
RRID
AB_370188
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
E2F transcription factor 1 , E2F-1 , RBAP1 , RBBP3 , RBP3
Cellular Localization
Nucleus
Background
The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F2 and E2F3, have an additional cyclin binding domain. This protein binds preferentially to retinoblastoma protein pRB in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent/independent apoptosis. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
|
GTX70167 WB Image
Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with E2F1 antibody [6B5] (GTX70167) diluted at 1:500. The signal was developed with Trident ECL plus-Enhanced.
|
|
GTX70167 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with E2F1 antibody [6B5] (GTX70167) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
|
REFERENCE
There are currently no references for E2F1 antibody [6B5] (GTX70167). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for E2F1 antibody [6B5] (GTX70167). Be the first to share your experience with this product.