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EDG7 antibody [N3C2], Internal

Orthogonal
Anti-EDG7 antibody [N3C2], Internal used in Western Blot (WB). GTX100018
Anti-EDG7 antibody [N3C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX100018
Anti-EDG7 antibody [N3C2], Internal used in Western Blot (WB). GTX100018
Anti-EDG7 antibody [N3C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX100018
Anti-EDG7 antibody [N3C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX100018

Cat. No. GTX100018

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all EDG7 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

40 kDa. ( Note )

Positive Control

NT2D1 , PC-3

Predict Reactivity

Rat, Bovine, Chicken, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide encompassing a sequence within the Intracellular domain of human EDG7. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1240739

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

lysophosphatidic acid receptor 3 , EDG7 , Edg-7 , GPCR , HOFNH30 , LP-A3 , LPA3 , RP4-678I3

Cellular Localization

Cell membrane; Multi-pass membrane protein

Background

This gene encodes a member of the G protein-coupled receptor family, as well as the EDG family of proteins. This protein functions as a cellular receptor for lysophosphatidic acid and mediates lysophosphatidic acid-evoked calcium mobilization. This receptor couples predominantly to G(q/11) alpha proteins. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-EDG7 antibody [N3C2], Internal used in Western Blot (WB). GTX100018

GTX100018 WB Image

Orthogonal

Unboiled PC-3 whole cell and membrane extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with EDG7 antibody [N3C2], Internal (GTX100018) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. (WCE: whole cell extract; ME: membrane extract)

Anti-EDG7 antibody [N3C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX100018

GTX100018 IHC-P Image

EDG7 antibody [N3C2], Internal detects EDG7 protein at cytosol on mouse kidney by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
EDG7 antibody [N3C2], Internal (GTX100018) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-EDG7 antibody [N3C2], Internal used in Western Blot (WB). GTX100018

GTX100018 WB Image

EDG7 antibody [N3C2], Internal detects LPAR3 protein by Western blot analysis.
A. 30 μg NT2D1 whole cell lysate/extract
B. 30 μg PC-3 whole cell lysate/extract
10 % SDS-PAGE
EDG7 antibody [N3C2], Internal (GTX100018) dilution: 1:1000

Anti-EDG7 antibody [N3C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX100018

GTX100018 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human testis, using EDG7(GTX100018) antibody(20 μg/ml).
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-EDG7 antibody [N3C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX100018

GTX100018 IHC-P Image

EDG7 antibody [N3C2], Internal detects EDG7 protein at cytosol on mouse kidney by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
EDG7 antibody [N3C2], Internal (GTX100018) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

Application Reference

Yamauchi A et al. BMC Cancer 2017; 17 (1) : 234 Evaluation of pancreatic cancer cell migration with multiple parameters in vitro by using an optical real-time cell mobility assay device.
Application : WB
Reactivity : Human
Chen YH et al. J Proteomics 2012; 77 : 111-28 Effect of high glucose on secreted proteome in cultured retinal pigmented epithelium cells: its possible relevance to clinical diabetic retinopathy.
Application : WB
Reactivity : Human
Submit a Reference

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Document
Unmodified.pdf
Package List Price ($)
$ 399
$ 169