APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:1000-1:10000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
| Assay dependent |
Not tested in other applications.
Calculated MW
Positive Control
Neuro 2A , GL261 , PC-12 , HeLaS3 , Molt-4
Predict Reactivity
Bovine, Xenopus tropicalis, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS, 20% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human ERAB. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_1240764
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
hydroxysteroid 17-beta dehydrogenase 10 , 17b-HSD10 , ABAD , CAMR , DUPXp11.22 , ERAB , HADH2 , HCD2 , HSD10MD , MHBD , MRPP2 , MRX17 , MRX31 , MRXS10 , SCHAD , SDR5C1
Cellular Localization
Mitochondrion
Background
This gene encodes 3-hydroxyacyl-CoA dehydrogenase type II, a member of the short-chain dehydrogenase/reductase superfamily. The gene product is a mitochondrial protein that catalyzes the oxidation of a wide variety of fatty acids, alcohols, and steroids. The protein has been implicated in the development of Alzheimer's disease, and mutations in the gene are the cause of 2-methyl-3-hydroxybutyryl-CoA dehydrogenase deficiency (MHBD). Several alternatively spliced transcript variants have been identified, but the full-length nature of only two transcript variants has been determined. [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX100301 IHC-Wm Image
ERAB antibody detects ERAB protein on whole-mount zebrafish embryos by immunohistochemical analysis.
Sample: Paraformaldehyde-fixed zebrafish embryos.
ERAB antibody (GTX100301) dilution: 1:200.
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GTX100301 IHC-P Image
Immunohistochemical analysis of paraffin-embedded OVCA xenograft, using ERAB(GTX100301) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX100301 IHC-P Image
ERAB antibody detects ERAB protein at mitochondria on mouse intestine by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
ERAB antibody (GTX100301) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX100301 WB Image
Sample (30 ug of whole cell lysate)
A: Neuro 2A
B: GL261
12% SDS PAGE
GTX100301 diluted at 1:1000
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GTX100301 IHC-P Image
ERAB antibody detects ERAB protein at mitochondria on mouse prostate by immunohistochemical analysis.
Sample: Paraffin-embedded mouse prostate.
ERAB antibody (GTX100301) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX100301 WB Image
Sample (30 ug of whole cell lysate)
A: PC-12
12% SDS PAGE
GTX100301 diluted at 1:5000
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GTX100301 IHC-P Image
ERAB antibody detects ERAB protein at mitochondria on mouse intestine by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
ERAB antibody (GTX100301) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX100301 ICC/IF Image
ERAB antibody detects ERAB protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: ERAB protein stained by ERAB antibody (GTX100301) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.
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GTX100301 WB Image
Sample(30 μg of whole cell lysate)
A:HeLa S3(GTX14654)
B:MOLT4 (GTX27912)
15% SDS PAGE
GTX100301 diluted at 1:1000
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REFERENCE
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REVIEW
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