APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1/500 - 1/2000 |
| 1/200 - 1/1000 |
| 1/200 - 1/1000 |
| 1/200 - 1/400 |
| 1/10000 |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
Ascites
Preservative
0.03% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Antigen Species
Human
Immunogen
Purified recombinant fragment of human MAPK3 expressed in E. Coli.
Purification
Unpurified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
mitogen-activated protein kinase 3 , ERK-1 , ERK1 , ERT2 , HS44KDAP , HUMKER1A , P44ERK1 , P44MAPK , PRKM3 , p44-ERK1 , p44-MAPK
Cellular Localization
Cytoplasm,Nucleus
Background
The protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act in a signaling cascade that regulates various cellular processes such as proliferation, differentiation, and cell cycle progression in response to a variety of extracellular signals. This kinase is activated by upstream kinases, resulting in its translocation to the nucleus where it phosphorylates nuclear targets. Alternatively spliced transcript variants encoding different protein isoforms have been described. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
![Anti-ERK1 antibody [1E5] used in Western Blot (WB). GTX60492](/templates/default/js/lightbox/images/loading.gif "Anti-ERK1 antibody [1E5] used in Western Blot (WB). GTX60492")
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GTX60492 WB Image
WB analysis of human ERK1 (AA: 9-143) recombinant protein using GTX60492 ERK1 antibody [1E5].
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GTX60492 ICC/IF Image
ICC/IF analysis of NIH3T3 cells using GTX60492 ERK1 antibody [1E5].
Green : ERK1
Blue: DRAQ5 fluorescent DNA dye
Red: Actin filaments
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GTX60492 IHC-P Image
IHC-P analysis of bladder cancer tissue using GTX60492 ERK1 antibody [1E5].
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GTX60492 IHC-P Image
IHC-P analysis of breast cancer tissue using GTX60492 ERK1 antibody [1E5].
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GTX60492 FACS Image
FACS analysis of HeLa cells using GTX60492 ERK1 antibody [1E5].
Blue : ERK1
Red : negative control
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GTX60492 WB Image
WB analysis of Hela (1), Jurkat (2), RAW264.7 (3), HEK293 (4), K562 (5), NIH3T3 (6), Cos7 (7) and PC-12 (8) cell lysate using GTX60492 ERK1 antibody [1E5].
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GTX60492 ELISA Image
ELISA analysis of antigen using GTX60492 ERK1 antibody [1E5].
Red : Control antigen 100ng
Purple : Antigen 10ng
Green : Antigen 50ng
Blue : Antigen 100ng
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REFERENCE
There are currently no references for ERK1 antibody [1E5] (GTX60492). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for ERK1 antibody [1E5] (GTX60492). Be the first to share your experience with this product.
![Anti-ERK1 antibody [1E5] used in Western Blot (WB). GTX60492](/upload/website/prouct_img/small/GTX60492/GTX60492_20170912_WB_1_w_23061123_123.webp "Anti-ERK1 antibody [1E5] used in Western Blot (WB). GTX60492")
![Anti-ERK1 antibody [1E5] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60492](/upload/website/prouct_img/small/GTX60492/GTX60492_20170912_ICCIF_w_23061123_886.webp "Anti-ERK1 antibody [1E5] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60492")
![Anti-ERK1 antibody [1E5] used in IHC (Paraffin sections) (IHC-P). GTX60492](/upload/website/prouct_img/small/GTX60492/GTX60492_20170912_IHC-P_w_23061123_489.webp "Anti-ERK1 antibody [1E5] used in IHC (Paraffin sections) (IHC-P). GTX60492")
![Anti-ERK1 antibody [1E5] used in IHC (Paraffin sections) (IHC-P). GTX60492](/upload/website/prouct_img/small/GTX60492/GTX60492_20170912_IHC-P_1_w_23061123_976.webp "Anti-ERK1 antibody [1E5] used in IHC (Paraffin sections) (IHC-P). GTX60492")
![Anti-ERK1 antibody [1E5] used in Flow cytometry (FACS). GTX60492](/upload/website/prouct_img/small/GTX60492/GTX60492_20170912_FACS_w_23061123_826.webp "Anti-ERK1 antibody [1E5] used in Flow cytometry (FACS). GTX60492")
![Anti-ERK1 antibody [1E5] used in Western Blot (WB). GTX60492](/upload/website/prouct_img/small/GTX60492/GTX60492_20170912_WB_w_23061123_228.webp "Anti-ERK1 antibody [1E5] used in Western Blot (WB). GTX60492")
![Anti-ERK1 antibody [1E5] used in ELISA (ELISA). GTX60492](/upload/website/prouct_img/small/GTX60492/GTX60492_20170912_ELISA_w_23061123_152.webp "Anti-ERK1 antibody [1E5] used in ELISA (ELISA). GTX60492")
![Anti-ERK1 antibody [1E5] used in Western Blot (WB). GTX60492](/upload/website/prouct_img/normal/GTX60492/GTX60492_20170912_WB_1_w_23061123_123.webp "Anti-ERK1 antibody [1E5] used in Western Blot (WB). GTX60492")
![Anti-ERK1 antibody [1E5] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60492](/upload/website/prouct_img/normal/GTX60492/GTX60492_20170912_ICCIF_w_23061123_886.webp "Anti-ERK1 antibody [1E5] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60492")
![Anti-ERK1 antibody [1E5] used in IHC (Paraffin sections) (IHC-P). GTX60492](/upload/website/prouct_img/normal/GTX60492/GTX60492_20170912_IHC-P_w_23061123_489.webp "Anti-ERK1 antibody [1E5] used in IHC (Paraffin sections) (IHC-P). GTX60492")
![Anti-ERK1 antibody [1E5] used in IHC (Paraffin sections) (IHC-P). GTX60492](/upload/website/prouct_img/normal/GTX60492/GTX60492_20170912_IHC-P_1_w_23061123_976.webp "Anti-ERK1 antibody [1E5] used in IHC (Paraffin sections) (IHC-P). GTX60492")
![Anti-ERK1 antibody [1E5] used in Flow cytometry (FACS). GTX60492](/upload/website/prouct_img/normal/GTX60492/GTX60492_20170912_FACS_w_23061123_826.webp "Anti-ERK1 antibody [1E5] used in Flow cytometry (FACS). GTX60492")
![Anti-ERK1 antibody [1E5] used in Western Blot (WB). GTX60492](/upload/website/prouct_img/normal/GTX60492/GTX60492_20170912_WB_w_23061123_228.webp "Anti-ERK1 antibody [1E5] used in Western Blot (WB). GTX60492")
![Anti-ERK1 antibody [1E5] used in ELISA (ELISA). GTX60492](/upload/website/prouct_img/normal/GTX60492/GTX60492_20170912_ELISA_w_23061123_152.webp "Anti-ERK1 antibody [1E5] used in ELISA (ELISA). GTX60492")