APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500 - 1:1000 |
| 1:100 - 1:500 |
| 1:100 - 1:200 |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
0.42% Potassium Phosphate, 0.87% NaCl, 30% Glycerol
Preservative
0.01% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
Batch dependent (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the center region of Estrogen Receptor alpha. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
estrogen receptor 1 , ER , ESR , ESRA , ESTRR , Era , NR3A1
Cellular Localization
Cytoplasm,Cell membrane,Cell membrane; Single-pass type I membrane protein,Golgi apparatus
Background
This gene encodes an estrogen receptor, a ligand-activated transcription factor composed of several domains important for hormone binding, DNA binding, and activation of transcription. The protein localizes to the nucleus where it may form a homodimer or a heterodimer with estrogen receptor 2. Estrogen and its receptors are essential for sexual development and reproductive function, but also play a role in other tissues such as bone. Estrogen receptors are also involved in pathological processes including breast cancer, endometrial cancer, and osteoporosis. Alternative promoter usage and alternative splicing result in dozens of transcript variants, but the full-length nature of many of these variants has not been determined. [provided by RefSeq, Mar 2014]
Database
Research Area
DATA IMAGES
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GTX32390 ICC/IF Image
ICC/IF analysis of formalin-fixed MCF7 cells using GTX32390 Estrogen Receptor alpha (phospho Ser106) antibody.
Red : Primary antibody
Blue : DAPI
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minutes
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GTX32390 WB Image
WB analysis of EGF-treated MCF7 (A) whole cell lysates using GTX32390 Estrogen Receptor alpha (phospho Ser106) antibody.
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GTX32390 IHC-P Image
IHC-P analysis of formalin fixed human breast cancer tissue section using GTX32390 Estrogen Receptor alpha (phospho Ser106) antibody.
Antigen retrieval : Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0)
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REFERENCE
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REVIEW
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