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FANCJ antibody [HL2961]

Recombinant antibody
Anti-FANCJ antibody [HL2961] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX640348
Anti-FANCJ antibody [HL2961] used in Western Blot (WB). GTX640348

Cat. No. GTX640348

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL2961

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all FANCJ products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
Not tested in other applications.

Calculated MW

141 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fragment of human FANCJ.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

BRCA1 interacting protein C-terminal helicase 1 , BACH1 , FANCJ , OF

Cellular Localization

Nucleus,Cytoplasm

Background

The protein encoded by this gene is a member of the RecQ DEAH helicase family and interacts with the BRCT repeats of breast cancer, type 1 (BRCA1). The bound complex is important in the normal double-strand break repair function of breast cancer, type 1 (BRCA1). This gene may be a target of germline cancer-inducing mutations. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-FANCJ antibody [HL2961] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX640348

GTX640348 ICC/IF Image

FANCJ antibody [HL2961] detects FANCJ protein by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: FANCJ stained by FANCJ antibody [HL2961] (GTX640348) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.

Anti-FANCJ antibody [HL2961] used in Western Blot (WB). GTX640348

GTX640348 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with FANCJ antibody [HL2961] (GTX640348) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations