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FGFR2 antibody

Anti-FGFR2 antibody used in IHC (Paraffin sections) (IHC-P). GTX10648
Anti-FGFR2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX10648

Cat. No. GTX10648

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse
Package
50 μl ($399)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:2,000
ICC/IF Assay dependent
IHC-P 1:1,000
Not tested in other applications.

Calculated MW

92 kDa. ( Note )

Product Note

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA

Preservative

15mM Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

synthetic peptide corresponding to amino acids 362-374 of the extracellular region of human FGFR-2 (Bek).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

RRID

AB_374021

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

fibroblast growth factor receptor 2 , BBDS , BEK , BFR-1 , CD332 , CEK3 , CFD1 , ECT1 , JWS , K-SAM , KGFR , TK14 , TK25

Cellular Localization

Cell membrane; Single-pass type I membrane protein,Golgi apparatus,Cytoplasmic vesicle

Background

Fibroblast growth factors (FGFs) are members of a large family of structurally related heparin binding polypeptides (17-38kD) that are potent physiological regulators of growth and differentiation for a wide variety of cells of mesodermal, ectodermal and endodermal origin. FGFs are substantially involved in normal development, wound healing and repair, angiogenesis, a variety of neurotrophic activities, in hematopoiesis as well as in tissue remodeling and maintenance. They have also been implicated in pathological conditions such as tumorigenesis and metastasis. The FGF family consists of at least seventeen members designated FGF1 through FGF17. To date, four genes encoding for high affinity cell surface FGF receptors (FGFRs) have been identified: FGFR1 [flg, cek1], FGFR2 [bek, cek3], FGFR3 [cek2] and FGFR4. Multiple additional variants (isoforms) arising by alternative splicing have been reported. Soluble, secreted or possibly cleaved forms of FGFR1 and FGFR2 have also been found in body fluids or were artificially constructed. FGFRs are members of the tyrosine kinase family of growth factor receptors. They are glycosylated 110- 150 kD proteins that are constructed of an extracellular ligand binding region with either two or typically three immunoglobulin (Ig)-like domains and an eight amino acid eacidic boxi, a transmembrane region and a cytoplasmic split tyrosine kinase domain that is activated following ligand binding and receptor dimerization. The ligand binding site of all FGFRs is confined to the extracellular Ig-like domains 2 and 3. FGFRs exhibit overlapping recognition and redundant specificity. One receptor type may bind several of the FGFs with a similar affinity. Also one FGF type may bind similarly to several distinct receptors. This accounts for the rather identical effects of different FGF ligands on common cell types. FGFs binding to cellular FGFRs depends on, or is markedly facilitated by the low-affinity interaction of FGFs with the polysaccharide component of cell surface or extracellular matrix heparan sulfate proteoglycans (HSPG). For example, perlecan, a basement membrane HSPG, promotes high affinity binding of FGF2 in vitro and angiogenesis in vivo. Signal transduction by FGFRs requires dimerization or oligomerization and autophosphorylation of the receptors through their tyrosine kinase domain. Subsequent association with cytoplasmic signaling molecules leads to DNA synthesis or differentiation. The signaling and biological responses elicited by distinct FGFRs substantially differ and are dictated by the intracellular domain. At the mRNA level, FGFR2 is highly expressed in developing human tissues including the brain (preferentially in glial cells), choroid plexus, skin, lung, kidney and bone. It is widely expressed in many adult human and animal tissues. It may be found in several anchorage-dependent cells, such as normal and malignant breast cancer cells. Crouzon as well as other craniosynostosis syndromes (e.g. Pfeifferis, Apertis, Jackson-Weissi), disorders of human skeletal development, have been shown to be the result of mutations in the extracellular domain of FGFR2.

Database

Research Area

DATA IMAGES

Anti-FGFR2 antibody used in IHC (Paraffin sections) (IHC-P). GTX10648

GTX10648 IHC-P Image

IHC-P analysis of human heart tissue using GTX10648 FGFR2 antibody at 1:500.

Anti-FGFR2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX10648

GTX10648 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX10648 FGFR2 antibody at 1:500(red) with DAPI(blue). Cells were fixed and permeabilized with methanol followed by methanol:acetone.

REFERENCE

REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399