APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
0.03-0.1μg/ml |
5μg/ml |
Not tested in other applications.
Calculated MW
Positive Control
The peptide used to generate this antibody is available for purchase (GTX89512-PEP).
Product Note
This antibody is expected to recognize isoform a (NP_000177.2) only.
PROPERTIES
Form
Liquid
Buffer
TBS, 0.5% BSA
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.50 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Peptide with sequence C-HLVPDRKKDQYK, from the internal region of the protein sequence according to NP_000177.2.
Purification
Purified by ammonium sulphate precipitation followed by antigen affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
complement factor H , AHUS1 , AMBP1 , ARMD4 , ARMS1 , CFHL3 , FH , FHL1 , HF , HF1 , HF2 , HUS
Cellular Localization
Secreted
Background
This gene is a member of the Regulator of Complement Activation (RCA) gene cluster and encodes a protein with twenty short consensus repeat (SCR) domains. This protein is secreted into the bloodstream and has an essential role in the regulation of complement activation, restricting this innate defense mechanism to microbial infections. Mutations in this gene have been associated with hemolytic-uremic syndrome (HUS) and chronic hypocomplementemic nephropathy. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Oct 2011]
Database
Research Area
DATA IMAGES
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GTX89512 IHC-P Image
IHC-P analysis of human liver using GTX89512 Factor H antibody, Internal. Antigen retrieval : citrate buffer pH 6 Dilution : 5μg/ml
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GTX89512 WB Image
WB analysis of human lung lysate using GTX89512 Factor H antibody, Internal. Dilution : 0.03μg/ml Loading : 35μg protein in RIPA buffer
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REFERENCE
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REVIEW
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