APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 0.1-0.3μg/ml |
| 3.75μg/ml |
Not tested in other applications.
Calculated MW
Positive Control
The peptide used to generate this antibody is available for purchase (GTX88349-PEP).
Product Note
This antibody is expected to recognize reported isoforms 1 (NP_000034.1), 2 (NP_690610.1) and 3 (NP_690611.1).
PROPERTIES
Form
Liquid
Buffer
TBS, 0.5% BSA
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.50 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Peptide with sequence KTCRKHRKENQGSH, from the internal region of the protein sequence according to NP_000034.1; NP_690610.1; NP_690611.1.
Purification
Purified by ammonium sulphate precipitation followed by antigen affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
Fas cell surface death receptor , ALPS1A , APO-1 , APT1 , CD95 , FAS1 , FASTM , TNFRSF6
Cellular Localization
Cell membrane
Background
The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor contains a death domain. It has been shown to play a central role in the physiological regulation of programmed cell death, and has been implicated in the pathogenesis of various malignancies and diseases of the immune system. The interaction of this receptor with its ligand allows the formation of a death-inducing signaling complex that includes Fas-associated death domain protein (FADD), caspase 8, and caspase 10. The autoproteolytic processing of the caspases in the complex triggers a downstream caspase cascade, and leads to apoptosis. This receptor has been also shown to activate NF-kappaB, MAPK3/ERK1, and MAPK8/JNK, and is found to be involved in transducing the proliferating signals in normal diploid fibroblast and T cells. Several alternatively spliced transcript variants have been described, some of which are candidates for nonsense-mediated mRNA decay (NMD). The isoforms lacking the transmembrane domain may negatively regulate the apoptosis mediated by the full length isoform. [provided by RefSeq, Mar 2011]
Database
Research Area
DATA IMAGES
|
GTX88349 IHC-P Image
IHC-P analysis of human small intestine using GTX88349 Fas antibody, Internal.
Antigen retrieval : citrate buffer pH 6
Dilution : 3.75μg/ml
|
|
|
GTX88349 WB Image
WB analysis of Molt-4 cell lysate using GTX88349 Fas antibody, Internal.
Dilution : 0.1μg/ml
Loading : 35μg protein in RIPA buffer
|
|
|
GTX88349 IHC-P Image
IHC-P analysis of human tonsil using GTX88349 Fas antibody, Internal.
Antigen retrieval : citrate buffer pH 6
|
REFERENCE
There are currently no references for Fas antibody, Internal (GTX88349). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Fas antibody, Internal (GTX88349). Be the first to share your experience with this product.
