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GFP antibody (TxRd)

Anti-GFP antibody (TxRd) used in Western Blot (WB). GTX26660

Cat. No. GTX26660

Host

 Goat

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, ELISA

Reactivity

 Species independent
Package
100 μg ($399)
See all GFP products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB >1:10000
ICC/IF 1:500 - 1:2500
ELISA >1:20000
Not tested in other applications.

Product Note

No reaction was observed against Human, Mouse and Rat Serum Proteins.

PROPERTIES

Form

Liquid

Buffer

20mM Potassium Phosphate, 150mM NaCl, 1% BSA

Preservative

0.01% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. Protect from light.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Jellyfish

Immunogen

Recombinant Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria.

Purification

Purified by antigen-affinity chromatography.
From serum

Conjugation

Texas Red (TxRd)
Ratio : 4.2 molecules Texas Red per Goat IgG molecule.

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Background

Designed to detect GFP and its variants in ELISA (sandwich or capture), immunoblotting and immunoprecipitation Fluorochrome conjugated anti-GFP was assayed by immunofluorescence microscopy on prokaryotic (E. Coli) and eukaryotic (CHO cells) expression systems and was shown to detect GFP containing inserts. Significant amplification of signal was detected using fluorochrome conjugated anti-GFP relative to the fluorescence of GFP alone.

Research Area

DATA IMAGES

Anti-GFP antibody (TxRd) used in Western Blot (WB). GTX26660

GTX26660 WB Image

REFERENCE

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399