APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
Assay dependent |
Assay dependent |
Assay dependent |
Not tested in other applications.
Calculated MW
Product Note
Based on internal testing result, this antibody does not cross react with human GOT2.
PROPERTIES
Form
Liquid
Buffer
PBS, 10% Glycerol
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
The clone AT3G11 is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human GOT3 protein.
Purification
Protein A Purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
glutamic-oxaloacetic transaminase 1 , AST1 , ASTQTL1 , GIG18 , GOT1 , cAspAT , cCAT , glutamicoxaloacetic transaminase 1 , Aspartate Aminotransferase
Cellular Localization
Cytoplasm
Background
Glutamic-oxaloacetic transaminase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
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GTX57691 WB Image
WB analysis of various samples using GTX57691 GOT1 antibody. Lane 1 : Recombinant Human GOT1 protein Lane 2 : Recombinant Human GOT2 protein Loading : 100 ng Dilution : 1:1000
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GTX57691 FACS Image
FACS analysis of Hep3B cells using GTX57691 GOT1 antibody. Cell Number: 1 x 10⁶ cells Primary antibody: Red line Antibody amount: 2-5 μg
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GTX57691 ICC/IF Image
ICC/IF analysis of A549 cells using GTX57691 GOT1 antibody. Blue: DAPI Green: Primary antibody Dilution: 1:100
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GTX57691 ICC/IF Image
ICC/IF analysis of HeLa cells using GTX57691 GOT1 antibody. Blue: DAPI Green: Primary antibody Dilution: 1:100
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GTX57691 FACS Image
FACS analysis of HeLa cells using GTX57691 GOT1 antibody. Cell Number: 1 x 10⁶ cells Primary antibody: Red line Antibody amount: 2-5 μg
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GTX57691 WB Image
WB analysis of A427 whole cell lysate using GTX57691 GOT1 antibody. Loading : 40 μg Dilution : 1:1000
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GTX57691 WB Image
WB analysis of various samples using GTX57691 GOT1 antibody. Lane 1 : 293T cell lysate
Lane 2 : GOT1 transfected 293T cell lysate Loading : 10 μg Dilution : 1:1000
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REFERENCE
There are currently no references for GOT1 antibody [AT3G11] (GTX57691). Be the first to share your publications with this product.
REVIEW
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