APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1-2μg/ml for 30 minutes at RT |
Note :
IHC-P
Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95ºC followed by cooling at RT for 20 minutes.
|
Not tested in other applications.
Calculated MW
Product Note
This MAb is highly specific to grp94 and shows minimal cross-reaction with other members of the HSP90 family.
PROPERTIES
Form
Liquid
Buffer
PBS, 0.05% BSA
Preservative
0.05% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.2 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant full-length human HSP90B1 protein
Purification
Protein A/G purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
ECGP , GP96 , GRP94 , HEL35 , HELS125m , HSP90B1 , TRA1 , heat shock protein 90 beta family member 1 , gp96 , ERp99 , Endoplasmin
Cellular Localization
Endoplasmic reticulum lumen,Melanosome
Background
This gene encodes a member of a family of adenosine triphosphate(ATP)-metabolizing molecular chaperones with roles in stabilizing and folding other proteins. The encoded protein is localized to melanosomes and the endoplasmic reticulum. Expression of this protein is associated with a variety of pathogenic states, including tumor formation. There is a microRNA gene located within the 5' exon of this gene. There are pseudogenes for this gene on chromosomes 1 and 15. [provided by RefSeq, Aug 2012]
Database
Research Area
DATA IMAGES
|
GTX34773 IHC-P Image
IHC-P analysis of human breast carcinoma tissue using GTX34773 GRP94 antibody [HSP90B1/1192].
|
REFERENCE
There are currently no references for GRP94 antibody [HSP90B1/1192] (GTX34773). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for GRP94 antibody [HSP90B1/1192] (GTX34773). Be the first to share your experience with this product.