APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
Assay dependent |
Assay dependent |
Assay dependent |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS, 10% Glycerol
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
The clone AT12C10 is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human GSTP3 protein.
Purification
Protein A Purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
glutathione S-transferase pi 1 , DFN7 , FAEES3 , GST3 , GSTP , GSTP1 , HELS22 , PI , glutathione Stransferase pi 1 , GST pi
Cellular Localization
Cytoplasm,Mitochondrion,Nucleus
Background
Glutathione S-transferases (GSTs) are a family of enzymes that play an important role in detoxification by catalyzing the conjugation of many hydrophobic and electrophilic compounds with reduced glutathione. Based on their biochemical, immunologic, and structural properties, the soluble GSTs are categorized into 4 main classes: alpha, mu, pi, and theta. This GST family member is a polymorphic gene encoding active, functionally different GSTP1 variant proteins that are thought to function in xenobiotic metabolism and play a role in susceptibility to cancer, and other diseases. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
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GTX57728 WB Image
WB analysis of various samples using GTX57728 GSTP1 antibody. Lane 1 : K562 whole cell lysate Lane 2 : Jurkat whole cell lysate Lane 3 : HeLa whole cell lysate Lane 4 : 293T whole cell lysate Lane 5 : A549 whole cell lysate Loading : 40 μg Dilution : 1:1000
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GTX57728 FACS Image
FACS analysis of K562 cells using GTX57728 GSTP1 antibody. Cell Number: 1 x 10⁶ cells Primary antibody: Red line Antibody amount: 2-5 μg
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GTX57728 ICC/IF Image
ICC/IF analysis of A549 cells using GTX57728 GSTP1 antibody. Blue: DAPI Green: Primary antibody Dilution: 1:100
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REFERENCE
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REVIEW
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