APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
0.01-0.03μg/ml |
5μg/ml |
Not tested in other applications.
Calculated MW
Positive Control
The peptide used to generate this antibody is available for purchase (GTX89536-PEP).
Predict Reactivity
Mouse, Rat, Bovine, Dog(>80% identity)
PROPERTIES
Form
Liquid
Buffer
TBS, 0.5% BSA
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.50 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Peptide with sequence C-LADQGQSWKEEV, from the internal region of the protein sequence according to NP_000843.1.
Purification
Purified by ammonium sulphate precipitation followed by antigen affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
glutathione S-transferase pi 1 , DFN7 , FAEES3 , GST3 , GSTP , HEL-S-22 , PI
Cellular Localization
Cytoplasm , Mitochondrion , Nucleus
Background
Glutathione S-transferases (GSTs) are a family of enzymes that play an important role in detoxification by catalyzing the conjugation of many hydrophobic and electrophilic compounds with reduced glutathione. Based on their biochemical, immunologic, and structural properties, the soluble GSTs are categorized into 4 main classes: alpha, mu, pi, and theta. This GST family member is a polymorphic gene encoding active, functionally different GSTP1 variant proteins that are thought to function in xenobiotic metabolism and play a role in susceptibility to cancer, and other diseases. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
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GTX89536 WB Image
WB analysis of human kidney lysate using GTX89536 GSTP1 antibody, Internal. Dilution : 0.01μg/ml Loading : 35μg protein in RIPA buffer
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GTX89536 WB Image
WB analysis of HEK293 overexpressing GSTP1 (mock transfection in first lane) using GTX89536 GSTP1 antibody, Internal.
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GTX89536 IHC-P Image
IHC-P analysis of human prostate using GTX89536 GSTP1 antibody, Internal. Antigen retrieval : citrate buffer pH 6 Dilution : 5μg/ml
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REFERENCE
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REVIEW
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