APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
0.2-2.5 ug/ml |
Not tested in other applications.
Calculated MW
Positive Control
ACHN , kidney proximal tubules from cortex , LLCPK1-F+pig kidney proximal tubule , rat brain , rat kidney cortex
Product Note
Specific for KGA but not GAC isoform
PROPERTIES
Form
Liquid
Buffer
PBS, 2% Sucrose
Preservative
0.09% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.5-1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
A synthetic peptide corresponding to a C-terminal region of Human GLS
Purification
Affinity Purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
glutaminase , AAD20 , CASGID , EIEE71 , GAC , GAM , GLS1 , KGA
Background
This gene encodes the K-type mitochondrial glutaminase. The encoded protein is an phosphate-activated amidohydrolase that catalyzes the hydrolysis of glutamine to glutamate and ammonia. This protein is primarily expressed in the brain and kidney plays an essential role in generating energy for metabolism, synthesizing the brain neurotransmitter glutamate and maintaining acid-base balance in the kidney. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jan 2012]
Database
Research Area
DATA IMAGES
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GTX50891 WB Image
WB analysis of rat kidney cortex, kidney proximal tubules from cortex, rat brain tissue, and pig LLCPK1-F cells using GTX50891 GLS antibody at 1:2000.
Lane 5 : rat kidney cortex
Lane 6 : rat kidney proximal tubules from cortex
Lane 7 : pig LLCPK1-F cells
Lane 8 : rat brain
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GTX50891 WB Image
WB analysis of ACHN cells using GTX50891 GLS antibody at 0.2-1μg/ml.
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REFERENCE
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REVIEW
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