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Glypican-3 antibody [GT764]

Orthogonal
Anti-Glypican-3 antibody [GT764] used in Western Blot (WB). GTX633303
Anti-Glypican-3 antibody [GT764] used in Flow cytometry (FACS). GTX633303

Cat. No. GTX633303

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 GT764

Isotype

 IgG2b

Application

 WB, FACS

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all Glypican-3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
FACS 1:50-1:200
Not tested in other applications.

Calculated MW

66 kDa. ( Note )

Positive Control

HepG2 , HepG2 (24 μg/ml Tunicamycin treatment for 16 hr)

Predict Reactivity

Bovine, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human Glypican-3. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2888335

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

glypican 3 , DGSX , GTR2-2 , MXR7 , OCI-5 , SDYS , SGB , SGBS , SGBS1

Cellular Localization

Cell membrane; Lipid-anchor , GPI-anchor; Extracellular side , Secreted glypican-3: Secreted , extracellular space

Background

Cell surface heparan sulfate proteoglycans are composed of a membrane-associated protein core substituted with a variable number of heparan sulfate chains. Members of the glypican-related integral membrane proteoglycan family (GRIPS) contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol linkage. These proteins may play a role in the control of cell division and growth regulation. Deletion mutations in this gene are associated with Simpson-Golabi-Behmel syndrome. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Glypican-3 antibody [GT764] used in Western Blot (WB). GTX633303

GTX633303 WB Image

Orthogonal

Untreated (–) and treated (+) HepG2 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Glypican-3 antibody [GT764] (GTX633303) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-Glypican-3 antibody [GT764] used in Flow cytometry (FACS). GTX633303

GTX633303 FACS Image

Glypican-3 antibody [GT764] (GTX633303) detects Glypican-3 protein by flow cytometry analysis.
Sample: HepG2 cell.
Black: Unlabelled sample was used as a control.
Red: Glypican-3 antibody [GT764] (GTX633303) dilution: 1:50.
Acquisition of 20,000 events were collected for FACS analysis.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 399
$ 169