APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:20,000 |
| Assay dependent |
| 1:500 |
| 5-10 μl |
Not tested in other applications.
Calculated MW
Positive Control
HeLa nuclear extract, human lymph node, NIH3T3
Product Note
Solution in 0.01 M phosphate bufffered saline, pH 7.4, containing 15 mM sodium azide.
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
15mM Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
Batch dependent (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
synthetic peptide corresponding to amino acids 466-482 of human and mouse HDAC1.
Purification
IgG fraction
Conjugation
Unconjugated
RRID
AB_371565
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
histone deacetylase 1 , GON-10 , HD1 , KDAC1 , RPD3 , RPD3L1
Cellular Localization
Nucleus
Background
Reversible acetylation of highly conserved lysine residues in N-terminal tail domains of core histones plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone acetylation is a dynamic process determined by the net activities of histone acetyltransferases (HATs) and the competing enzymes histone deacetylases (HDACs) (ref 3). Histone deacetylases activities were often, but not always, associated with transcriptional repression and nucleosomal condensations (ref 6,7). Recruitment of the multiprotein complexes to promoter sites occurs by many sequence specific DNA-binding proteins such as unliganded nuclear hormone receptors, DP1-E2F, YY1 and Rb family of transcription factors, transcriptional repressors and tumor suppressors (e.g. BRCA1). Aberrant recruitment of HDACs by certain oncoproteins may occur in certain neoplastic diseases.(ref 11)
Database
Research Area
DATA IMAGES
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GTX27028 IP Image
IP analysis of HeLa cell lysate using GTX27028 HDAC1 antibody.
Lane 1 : IP from HeLa cell lysate using 5 μL antibody
Lane 2 : IP from HeLa cell lysate using 10 μL antibody
Lane 3 : IP from HeLa cell lysate using 10 μL non-relevant antibody
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GTX27028 WB Image
WB analysis of (1) HEK-293T (2) HeLa (3) K562 (4) CHO (5) NIH-3T3 (6) PC-12 cell lysate using GTX27028 HDAC1 antibody at 1:10,000.
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GTX27028 ICC/IF Image
ICC/IF analysis of HeLa cells using GTX27028 HDAC1 antibody at 1:200. Cells were fixed and permeabilized with cold methanol followed by cold methanol:acetone.
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REFERENCE
REVIEW
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