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HDAC3 antibody [GT9080]

Anti-HDAC3 antibody [GT9080] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60832
Anti-HDAC3 antibody [GT9080] used in ChIP assay (ChIP assay). GTX60832

Cat. No. GTX60832

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 GT9080

Isotype

 IgG

Application

 ICC/IF, ChIP assay

Reactivity

 Human
Package
50 μl ($399)
See all HDAC3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
ICC/IF Assay dependent
ChIP assay Assay dependent
Not tested in other applications.

Calculated MW

49 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Tissue culture supernatant

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Human HDAC3 (Histone deacetylase 3), using a KLH-conjugated synthetic peptide containing a sequence from the C-terminal region of the protein.

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

histone deacetylase 3 , HD3 , KDAC3 , RPD3 , RPD3-2

Cellular Localization

Nucleus , Cytoplasm , Cytoplasm, cytosol

Background

Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to the histone deacetylase/acuc/apha family. It has histone deacetylase activity and represses transcription when tethered to a promoter. It may participate in the regulation of transcription through its binding with the zinc-finger transcription factor YY1. This protein can also down-regulate p53 function and thus modulate cell growth and apoptosis. This gene is regarded as a potential tumor suppressor gene. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-HDAC3 antibody [GT9080] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60832

GTX60832 ICC/IF Image

ICC/IF analysis of HeLa cells using HDAC3 antibody (red) and DAPI. Cells were fixed with 4% formaldehyde for 10’ and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labelled with the HDAC3 antibody at a dilution of 1:500.

Anti-HDAC3 antibody [GT9080] used in ChIP assay (ChIP assay). GTX60832

GTX60832 ChIP assay Image

ChIP was performed with HeLa extracts and either 5 μg of HDAC3 antibody or control IgG. The precipitated DNA was detected by qPCR with primers targeting to GAPDH promoter, c-fos promoter and the coding region of p21, a known target gene of HDAC3.

REFERENCE

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REVIEW

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Package List Price ($)
$ 399