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HMGB1 + HMGB2 antibody

KO/KD
Anti-HMGB1 + HMGB2 antibody used in Western Blot (WB). GTX127972
Anti-HMGB1 + HMGB2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX127972
Anti-HMGB1 + HMGB2 antibody used in Western Blot (WB). GTX127972
Anti-HMGB1 + HMGB2 antibody used in IHC (Paraffin sections) (IHC-P). GTX127972
Anti-HMGB1 + HMGB2 antibody used in Western Blot (WB). GTX127972
Anti-HMGB1 + HMGB2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX127972
Anti-HMGB1 + HMGB2 antibody used in IHC (Paraffin sections) (IHC-P). GTX127972
Anti-HMGB1 + HMGB2 antibody used in Western Blot (WB). GTX127972

Cat. No. GTX127972

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all HMGB1 + HMGB2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:20000
ICC/IF 1:100-1:2000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

24 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , A375 , mouse brain , rat brain

Predict Reactivity

Bovine, Chicken, Pig, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human HMGB1 + HMGB2 Recombinant protein.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2885667

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Cellular Localization

Nucleus

Research Area

DATA IMAGES

Anti-HMGB1 + HMGB2 antibody used in Western Blot (WB). GTX127972

GTX127972 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with HMGB1/HMGB2 antibody (GTX127972) diluted at 1:20000.

Anti-HMGB1 + HMGB2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX127972

GTX127972 ICC/IF Image

HMGB1/HMGB2 antibody detects HMGB1/HMGB2 protein at nucleus by immunofluorescent analysis.
Sample: A375 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: HMGB1/HMGB2 protein stained by HMGB1/HMGB2 antibody (GTX127972) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-HMGB1 + HMGB2 antibody used in Western Blot (WB). GTX127972

GTX127972 WB Image

Rat tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with HMGB1/HMGB2 antibody (GTX127972) diluted at 1:2000.

Anti-HMGB1 + HMGB2 antibody used in IHC (Paraffin sections) (IHC-P). GTX127972

GTX127972 IHC-P Image

HMGB1/HMGB2 antibody detects HMGB2 protein at nucleus on human duodenal adenocarcinoma tissue by immunohistochemical analysis.
Sample: Paraffin-embedded human duodenal adenocarcinoma tissue.
HMGB1/HMGB2 antibody (GTX127972) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-HMGB1 + HMGB2 antibody used in Western Blot (WB). GTX127972

GTX127972 WB Image

HMGB1/HMGB2 antibody detects HMGB2 protein by Western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg A431 whole cell lysate/extract
C. 30 μg HeLa whole cell lysate/extract
D. 30 μg HepG2 whole cell lysate/extract
E. 30 μg A375 whole cell lysate/extract
12 % SDS-PAGE
HMGB1/HMGB2 antibody (GTX127972) dilution: 1:10000

Anti-HMGB1 + HMGB2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX127972

GTX127972 ICC/IF Image

HMGB1/HMGB2 antibody detects HMGB1/HMGB2 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: HMGB1/HMGB2 protein stained by HMGB1/HMGB2 antibody (GTX127972) diluted at 1:2000.
Red: phalloidin, a cytoskeleton marker, diluted at 1:200.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-HMGB1 + HMGB2 antibody used in IHC (Paraffin sections) (IHC-P). GTX127972

GTX127972 IHC-P Image

HMGB1/HMGB2 antibody detects HMGB2 protein at cytosol on mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
HMGB1/HMGB2 antibody (GTX127972) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-HMGB1 + HMGB2 antibody used in Western Blot (WB). GTX127972

GTX127972 WB Image

Mouse tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with HMGB1/HMGB2 antibody (GTX127972) diluted at 1:1000.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169