HP1 alpha antibody, Internal

Cat. No. GTX89432

Host	Goat
Clonality	Polyclonal
Isotype	IgG
Application	WB, IHC-P, FACS
Reactivity	Human, Mouse
Package	100 μg ($399)

See all HP1 alpha products

Datasheet
Document

View product citations for antibody GTX89432 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	0.03-0.1μg/ml
IHC-P	2-4μg/ml
FACS	10μg/ml
Note : IHC-P Human Uterus shows nuclear staining in the epithelial cells of the endometrium.

Not tested in other applications.

Calculated MW

22 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX89432-PEP).

Predict Reactivity

Dog(>80% identity)

PROPERTIES

Form

Liquid

Buffer

TBS, 0.5% BSA

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence C-NKRKSNFSNSADDIK, from the internal region of the protein sequence according to NP_036249.1; NP_001120793.1; NP_001120794.1.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

chromobox 5 , HEL25 , HP1 , HP1A

Cellular Localization

Nucleus

Background

This gene encodes a highly conserved nonhistone protein, which is a member of the heterochromatin protein family. The protein is enriched in the heterochromatin and associated with centromeres. The protein has a single N-terminal chromodomain which can bind to histone proteins via methylated lysine residues, and a C-terminal chromo shadow-domain (CSD) which is responsible for the homodimerization and interaction with a number of chromatin-associated nonhistone proteins. The encoded product is involved in the formation of functional kinetochore through interaction with essential kinetochore proteins. The gene has a pseudogene located on chromosome 3. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Jul 2008]

Database

Gene ID: 23468 CBX5
UniProt: P45973 CBX5

Research Area

DATA IMAGES

GTX89432 WB Image

WB analysis of K562 (A), MCF7 (B) and NIH-3T3 (C) cell lysate using GTX89432 HP1 alpha antibody, Internal.
Dilution : 0.1μg/ml
Loading : 35μg protein in RIPA buffer

GTX89432 FACS Image

FACS analysis of PFA fixed HeLa cells using GTX89432 HP1 alpha antibody, Internal.
Blue : Primary antibody
Black : Isotype control
Permeabilization : 0.5% Triton
Dilution : 10μg/ml

GTX89432 IHC-P Image

IHC-P analysis of human uterus using GTX89432 HP1 alpha antibody, Internal.
Antigen retrieval : citrate buffer pH 6
Dilution : 2μg/ml

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf

Package

List Price ($)

100 μg

$ 399

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