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HP1 gamma antibody [5G10]

Anti-HP1 gamma antibody [5G10] used in Western Blot (WB). GTX66848
Anti-HP1 gamma antibody [5G10] used in Immunoprecipitation (IP). GTX66848
Anti-HP1 gamma antibody [5G10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX66848
Anti-HP1 gamma antibody [5G10] used in IHC (Paraffin sections) (IHC-P). GTX66848

Cat. No. GTX66848

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 5G10

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human, Mouse, Rat, Hamster, Monkey
Package
100 μl ($459)
See all HP1 gamma products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000
ICC/IF 1:200
IHC-P 1:800
IP 1:50-1:200
Not tested in other applications.

Calculated MW

21 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

100mM Tris-Glycine, 150mM NaCl, 50% Glycerol

Preservative

0.2% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Purified recombinant human CBX3 protein fragments expressed in E.coli.

Purification

Purified by antigen-affinity chromatography

Purity

> 95% (by SDS-PAGE)

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

chromobox 3 , HECH , HP1-GAMMA , HP1Hs-gamma

Cellular Localization

Nucleus

Background

At the nuclear envelope, the nuclear lamina and heterochromatin are adjacent to the inner nuclear membrane. The protein encoded by this gene binds DNA and is a component of heterochromatin. This protein also can bind lamin B receptor, an integral membrane protein found in the inner nuclear membrane. The dual binding functions of the encoded protein may explain the association of heterochromatin with the inner nuclear membrane. This protein binds histone H3 tails methylated at Lys-9 sites. This protein is also recruited to sites of ultraviolet-induced DNA damage and double-strand breaks. Two transcript variants encoding the same protein but differing in the 5' UTR, have been found for this gene.[provided by RefSeq, Mar 2011]

Database

Research Area

DATA IMAGES

Anti-HP1 gamma antibody [5G10] used in Western Blot (WB). GTX66848

GTX66848 WB Image

WB analysis of HeLa, 3T3, C6, COS7, and CHO-K1 cell lysates using GTX66848 HP1 gamma antibody [5G10].
Dilution : 1:1000

Anti-HP1 gamma antibody [5G10] used in Immunoprecipitation (IP). GTX66848

GTX66848 IP Image

IP analysis of HeLa cell lysate using GTX66848 HP1 gamma antibody [5G10].

Anti-HP1 gamma antibody [5G10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX66848

GTX66848 ICC/IF Image

ICC/IF analysis of 4% PFA-fixed HeLa cells using GTX66848 HP1 gamma antibody [5G10].
Dilution : 1:200

Anti-HP1 gamma antibody [5G10] used in IHC (Paraffin sections) (IHC-P). GTX66848

GTX66848 IHC-P Image

IHC-P analysis of prostate cancer tissue using GTX66848 HP1 gamma antibody [5G10].
Antigen retrieval : citrate buffer (pH 6.0)

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 459