APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:1000-1:10000 |
| Assay dependent |
Not tested in other applications.
Calculated MW
Positive Control
HeLa (25 μg/ml Anisomycin treatment for 30 min)
Predict Reactivity
Bovine, Dog, Pig, Xenopus tropicalis, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Carrier-protein conjugated synthetic peptide surrounding phospho Ser82 of human HSP27. The exact sequence is proprietary.
Purification
Affinity purified by Protein A.
Conjugation
Unconjugated
RRID
AB_2888450
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
heat shock protein family B (small) member 1 , CMT2F , HEL-S-102 , HMN2B , HS.76067 , HSP27 , HSP28 , Hsp25 , SRP27
Cellular Localization
Cytoplasm , Nucleus , Spindle
Background
The protein encoded by this gene is induced by environmental stress and developmental changes. The encoded protein is involved in stress resistance and actin organization and translocates from the cytoplasm to the nucleus upon stress induction. Defects in this gene are a cause of Charcot-Marie-Tooth disease type 2F (CMT2F) and distal hereditary motor neuropathy (dHMN). [provided by RefSeq]
Database
Research Area
DATA IMAGES
![Anti-HSP27 (phospho Ser82) antibody [GT357] used in Western Blot (WB). GTX634421](/templates/default/js/lightbox/images/loading.gif "Anti-HSP27 (phospho Ser82) antibody [GT357] used in Western Blot (WB). GTX634421")
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GTX634421 WB Image
Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with HSP27 (phospho Ser82) antibody [GT357] (GTX634421) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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GTX634421 WB Image
Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with HSP27 (phospho Ser82) antibody [GT357] (GTX634421) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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GTX634421 WB Image
Untreated (–) and treated (+) C2C12 whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with HSP27 (phospho Ser82) antibody [GT357] (GTX634421) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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GTX634421 ICC/IF Image
HSP27 (phospho Ser82) antibody [GT357] detects HSP27 (phospho Ser82) protein at cytoplasm by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: HSP27 (phospho Ser82) stained by HSP27 (phospho Ser82) antibody [GT357] (GTX634421) diluted at 1:500.Blue: Hoechst 33342 staining.Scale bar= 10 μm.
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REFERENCE
There are currently no references for HSP27 (phospho Ser82) antibody [GT357] (GTX634421). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for HSP27 (phospho Ser82) antibody [GT357] (GTX634421). Be the first to share your experience with this product.
![Anti-HSP27 (phospho Ser82) antibody [GT357] used in Western Blot (WB). GTX634421](/upload/website/prouct_img/small/GTX634421/GTX634421_43024_20190809_WB_R_w_23061202_292.webp "Anti-HSP27 (phospho Ser82) antibody [GT357] used in Western Blot (WB). GTX634421")
![Anti-HSP27 (phospho Ser82) antibody [GT357] used in Western Blot (WB). GTX634421](/upload/website/prouct_img/small/GTX634421/GTX634421_43024_20171117_WB_Anisomycin_w_23061202_746.webp "Anti-HSP27 (phospho Ser82) antibody [GT357] used in Western Blot (WB). GTX634421")
![Anti-HSP27 (phospho Ser82) antibody [GT357] used in Western Blot (WB). GTX634421](/upload/website/prouct_img/small/GTX634421/GTX634421_43024_20190823_WB_treatment_M_CIP_w_23061202_951.webp "Anti-HSP27 (phospho Ser82) antibody [GT357] used in Western Blot (WB). GTX634421")
![Anti-HSP27 (phospho Ser82) antibody [GT357] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX634421](/upload/website/prouct_img/small/GTX634421/GTX634421_43024_20190717_ICC_IF_w_23061202_123.webp "Anti-HSP27 (phospho Ser82) antibody [GT357] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX634421")
![Anti-HSP27 (phospho Ser82) antibody [GT357] used in Western Blot (WB). GTX634421](/upload/website/prouct_img/normal/GTX634421/GTX634421_43024_20190809_WB_R_w_23061202_292.webp "Anti-HSP27 (phospho Ser82) antibody [GT357] used in Western Blot (WB). GTX634421")
![Anti-HSP27 (phospho Ser82) antibody [GT357] used in Western Blot (WB). GTX634421](/upload/website/prouct_img/normal/GTX634421/GTX634421_43024_20171117_WB_Anisomycin_w_23061202_746.webp "Anti-HSP27 (phospho Ser82) antibody [GT357] used in Western Blot (WB). GTX634421")
![Anti-HSP27 (phospho Ser82) antibody [GT357] used in Western Blot (WB). GTX634421](/upload/website/prouct_img/normal/GTX634421/GTX634421_43024_20190823_WB_treatment_M_CIP_w_23061202_951.webp "Anti-HSP27 (phospho Ser82) antibody [GT357] used in Western Blot (WB). GTX634421")
![Anti-HSP27 (phospho Ser82) antibody [GT357] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX634421](/upload/website/prouct_img/normal/GTX634421/GTX634421_43024_20190717_ICC_IF_w_23061202_123.webp "Anti-HSP27 (phospho Ser82) antibody [GT357] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX634421")