APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:500-1:3000 |
1:100-1:500 |
Not tested in other applications.
Positive Control
zebrafish heart , zebrafish muscle , whole zebrafish
PROPERTIES
Form
Liquid
Buffer
PBS, 20% Glycerol
Preservative
0.025% ProClin 300
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.54 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Zebrafish
Immunogen
Recombinant protein encompassing a sequence within the center region of zebrafish Hbae1. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_2885803
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
hemoglobin, alpha embryonic 1.1 , HBA2r , HBAA1 , SI:dZ118J2.10 , SI:dZ118J2.8 , dZ118J2.6 , hba2 , hbae1 , si:by119c3.1 , si:by119c3.3 , si:xx-119c3.3 , wu:fa12a11 , wu:fb74f06 , zgc:86936
Database
Research Area
DATA IMAGES
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GTX128636 IHC-Wm Image
Hbae1 antibody detects Hbae1 protein on whole mount zebrafish by immunohistochemical analysis.Sample: Paraformaldehyde-fixed 2 days-post-fertilization zebrafish embryo.Green: Hbae1 stained by Hbae1 antibody (GTX128636) diluted at 1:100.
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GTX128636 IHC-Wm Image
Hbae1 antibody detects Hbae1 protein on whole mount zebrafish by immunohistochemical analysis.Sample: Paraformaldehyde-fixed 2 days-post-fertilization zebrafish embryo.Green: Hbae1 stained by Hbae1 antibody (GTX128636) diluted at 1:100.
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GTX128636 WB Image
Various tissue extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Hbae1 antibody (GTX128636) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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REFERENCE
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REVIEW
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