Summary
GZMB produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 235 amino acids (19-247a.a.) and having a molecular mass of 26.5kDa. (Molecular size on SDS-PAGE will appear at approximately 28-40kDa). GZMB is expressed with an 6 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.
Form
Liquid
Buffer
PBS, 20% Glycerol, 1mM DTT
Preservative
No preservative
Storage
Store as concentrated solution. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, we recommend adding a carrier protein (0.1% HSA or BSA), then aliquot and store at -20ºC or below. Avoid freeze-thaw cycles.
Concentration
0.5 mg/ml (Please refer to the vial label for the specific concentration.)
Region/Sequence
C-terminal His-Tag; GEIIGGHEAK PHSRPYMAYL MIWDQKSLKR CGGFLIRDDF VLTAAHCWGS SINVTLGAHN IKEQEPTQQF IPVKRPIPHP AYNPKNFSND IMLLQLERKA KRTRAVQPLR LPSNKAQVKP GQTCSVAGWG QTAPLGKHSH TLQEVKMTVQ EDRKCESDLR HYYDSTIELC VGDPEIKKTS FKGDSGGPLV CNKVAQGIVS YGRNNGMPPR ACTKVSSFVH WIKKTMKRYH HHHHH
Expression System
Baculovirus (Sf9 cells)
Purity
??95% by SDS-PAGE.
Conjugation
Unconjugated
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Synonyms
granzyme B , C11 , CCPI , CGL-1 , CGL1 , CSP-B , CSPB , CTLA1 , CTSGL1 , HLP , SECT
Database
Research Area