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IL2 antibody (Biotin)

Anti-IL2 antibody (Biotin) used in ELISA (ELISA). GTX43856

Cat. No. GTX43856

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ELISA

Reactivity

Human
Package
50 μg ($589)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.1-0.2μg/ml
ELISA 0.25-1μg/ml
Not tested in other applications.

Calculated MW

18 kDa. ( Note )

Product Note

This antibody may be used in a direct ELISA or as the detection reagent in a sandwich ELISA with GTX39952.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

50 μg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant human IL-2

Purification

Purified by affinity chromatography

Conjugation

Biotin

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

interleukin 2 , IL-2 , TCGF , lymphokine

Cellular Localization

Secreted

Background

The protein encoded by this gene is a secreted cytokine that is important for the proliferation of T and B lymphocytes. The receptor of this cytokine is a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL4) and interleukin 7 (IL7). The expression of this gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a similar gene in mice leads to ulcerative colitis-like disease, which suggests an essential role of this gene in the immune response to antigenic stimuli. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-IL2 antibody (Biotin) used in ELISA (ELISA). GTX43856

GTX43856 ELISA Image

Sandwich ELISA analysis of human interleukin-2 using Rat anti Human IL-2 as a capture reagent and biotinylated Rabbit anti Human IL-2 (GTX43856) as a detection reagent. In the enlargement, neat serum (orange) and neat plasma (green) samples, low levels of human IL-2 (~1pg/ml) are detected.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 589