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IZUMO1 antibody [#125]

KO/KD Orthogonal
Anti-IZUMO1 antibody [#125] used in Western Blot (WB). GTX00689
Anti-IZUMO1 antibody [#125] used in Western Blot (WB). GTX00689
Anti-IZUMO1 antibody [#125] used in Immunoprecipitation (IP). GTX00689
Anti-IZUMO1 antibody [#125] used in Western Blot (WB). GTX00689
Anti-IZUMO1 antibody [#125] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00689

Cat. No. GTX00689

Reference 2

Host

 Rat

Clonality

 Monoclonal

Clone Name

 #125

Isotype

 IgG2a

Application

 WB, ICC/IF, IP

Reactivity

 Human, Mouse
Package
100 μg ($399)
See all IZUMO1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000
ICC/IF 1:250
IP 1:100
Not tested in other applications.

Calculated MW

45 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Filter-sterilized PBS, 50% Glycerol

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Mouse sperm

Purification

Purified IgG

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

izumo sperm-egg fusion 1 , 1700058F15Rik , Izumo

Cellular Localization

Cell membrane

Database

Research Area

DATA IMAGES

Anti-IZUMO1 antibody [#125] used in Western Blot (WB). GTX00689

GTX00689 WB Image

Orthogonal

WB analysis of various mouse tissue samples using GTX00689 IZUMO1 antibody [#125].
Dilution : 1:1000

Anti-IZUMO1 antibody [#125] used in Western Blot (WB). GTX00689

GTX00689 WB Image

WB analysis of human testis or sperm tissue lysate samples using GTX00689 IZUMO1 antibody [#125].
Dilution : 1:1000

Anti-IZUMO1 antibody [#125] used in Immunoprecipitation (IP). GTX00689

GTX00689 IP Image

IP analysis of mouse sperm lysate using GTX00689 IZUMO1 antibody [#125].
Lane 1 : Negative control without the antibody
Lane 2 : Primary antibody
Total protein amount : 1.5 mg
IP antibody : 10 μg
IB antibody : 10 μl
Total protein amount : 1.5 mg

Anti-IZUMO1 antibody [#125] used in Western Blot (WB). GTX00689

GTX00689 WB Image

KO/KD

WB analysis of mouse sperm using GTX00689 IZUMO1 antibody [#125].
Lane 1 : Wild-type mouse
Lane 2 : IZUMO1 knock-out mouse
Dilution : 1:1000
Loading : 10 μg

Anti-IZUMO1 antibody [#125] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00689

GTX00689 ICC/IF Image

ICC/IF analysis of mouse spermatozoa using GTX00689 IZUMO1 antibody [#125].
Green : Primary antibody
Dilution : 4 μg/ml
Fixation : ice-cold 100% ethanol

REFERENCE

Clone Reference

Inoue N et al. Development. 2013; 140 (15) Molecular dissection of IZUMO1, a sperm protein essential for sperm-egg fusion.
Application : WB, ICC/IF
Reactivity : Mouse
Inoue N et al. PLoS One.? 2010; 5 (4) Identification and disruption of sperm-specific angiotensin converting enzyme-3 (ACE3) in mouse.
Application : WB, ICC/IF, IP
Reactivity : Mouse
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Package List Price ($)
$ 399