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JNK antibody

Anti-JNK antibody used in Western Blot (WB). GTX04658
Anti-JNK antibody used in Western Blot (WB). GTX04658
Anti-JNK antibody used in IHC (Paraffin sections) (IHC-P). GTX04658
Anti-JNK antibody used in IHC (Paraffin sections) (IHC-P). GTX04658
Anti-JNK antibody used in IHC (Paraffin sections) (IHC-P). GTX04658
Anti-JNK antibody used in IHC (Paraffin sections) (IHC-P). GTX04658
Anti-JNK antibody used in IHC (Paraffin sections) (IHC-P). GTX04658
Anti-JNK antibody used in IHC (Paraffin sections) (IHC-P). GTX04658
Anti-JNK antibody used in Western Blot (WB). GTX04658
Anti-JNK antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX04658
Anti-JNK antibody used in Flow cytometry (FACS). GTX04658
Anti-JNK antibody used in Western Blot (WB). GTX04658
Anti-JNK antibody used in IHC (Paraffin sections) (IHC-P). GTX04658

Cat. No. GTX04658

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, FACS

Reactivity

 Human, Mouse, Rat, Chicken, Pig
Package
100 μl ($399)
See all JNK products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:300-1:5000
ICC/IF 1:50-1:200
IHC-P 1:200-1:400
FACS 1:20-1:100
Not tested in other applications.

Calculated MW

42-47 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

10mM TBS, 1% BSA, 50% Glycerol

Preservative

0.03% Proclin300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

KLH conjugated synthetic peptide derived from mouse JNK1/2/3(151-250)

Purification

Protein A purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

DATA IMAGES

Anti-JNK antibody used in Western Blot (WB). GTX04658

GTX04658 WB Image

WB analysis of mouse spleen tissue lysate using GTX04658 JNK antibody.
Dilution : 1:300

Anti-JNK antibody used in Western Blot (WB). GTX04658

GTX04658 WB Image

WB analysis of various sample lysates using GTX04658 JNK antibody.
Lane 1 : Mouse kidney tissue lysates
Lane 2 : Mouse cerebrum tissue lysate
Lane 3 : Rat cerebrum tissue lysate
Dilution : 1:1000

Anti-JNK antibody used in IHC (Paraffin sections) (IHC-P). GTX04658

GTX04658 IHC-P Image

IHC-P analysis of rat kidney tissue using GTX04658 JNK antibody.
Antigen retrieval : Boiled in sodium citrate buffer (pH6.0)
Dilution : 1:200

Anti-JNK antibody used in IHC (Paraffin sections) (IHC-P). GTX04658

GTX04658 IHC-P Image

IHC-P analysis of mouse brain tissue using GTX04658 JNK antibody.
Antigen retrieval : Boiled in sodium citrate buffer (pH6.0)
Dilution : 1:200

Anti-JNK antibody used in IHC (Paraffin sections) (IHC-P). GTX04658

GTX04658 IHC-P Image

IHC-P analysis of rat brain tissue using GTX04658 JNK antibody.
Antigen retrieval : Boiled in sodium citrate buffer (pH6.0)
Dilution : 1:200

Anti-JNK antibody used in IHC (Paraffin sections) (IHC-P). GTX04658

GTX04658 IHC-P Image

IHC-P analysis of rat brain tissue using GTX04658 JNK antibody.
Dilution : 1:200

Anti-JNK antibody used in IHC (Paraffin sections) (IHC-P). GTX04658

GTX04658 IHC-P Image

IHC-P analysis of mouse brain tissue using GTX04658 JNK antibody.
Antigen retrieval : Boiled in sodium citrate buffer (pH6.0)
Dilution : 1:200

Anti-JNK antibody used in IHC (Paraffin sections) (IHC-P). GTX04658

GTX04658 IHC-P Image

IHC-P analysis of mouse brain tissue using GTX04658 JNK antibody.
Antigen retrieval : Boiled in sodium citrate buffer (pH6.0)
Dilution : 1:400

Anti-JNK antibody used in Western Blot (WB). GTX04658

GTX04658 WB Image

WB analysis of various samples using GTX04658 JNK antibody.
Lane 1: Rat cerebrum tissue lysate
Lane 2: Mouse cerebrum tissue lysate
Lane 3: Rat heart tissue lysate
Lane 4: Mouse heart tissue lysate
Lane 5: Rat cerebrum tissue lysate
Lane 6: Mouse cerebrum tissue lysate
Dilution : 1:1000

Anti-JNK antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX04658

GTX04658 ICC/IF Image

ICC/IF analysis of 4% PFA-fixed HeLa cells using GTX04658 JNK antibody.
Green : Primary antibody
Blue : DAPI
Permeabilization : Triton X-100
Dilution : 1:100

Anti-JNK antibody used in Flow cytometry (FACS). GTX04658

GTX04658 FACS Image

FACS analysis of PFA-fixed HUVEC cells using GTX04658 JNK antibody.
Green : Primary antibody
Orange: Isotype control
Blue: Secondary antibody
Black: Cells only
Permeabilization : 90% ice-cold methanol
Dilution : 1:50

Anti-JNK antibody used in Western Blot (WB). GTX04658

GTX04658 WB Image

WB analysis of various samples using GTX04658 JNK antibody.
Lane 1: Mouse brain tissue lysate
Lane 2: Rat brain tissue lysate
Lane 3: Mouse cerebellum tissue lysate
Lane 4: Mouse heart tissue lysate
Dilution : 1:300

Anti-JNK antibody used in IHC (Paraffin sections) (IHC-P). GTX04658

GTX04658 IHC-P Image

IHC-P analysis of rat brain tissue using GTX04658 JNK antibody.
Antigen retrieval : Boiled in sodium citrate buffer (pH6.0)
Dilution : 1:200

REFERENCE

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Package List Price ($)
$ 399