LEF1 antibody

Cat. No. GTX129186

Reference 6

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, IHC-P
Reactivity	Human, Rat
Package	100 μl ($399), 25 μl ($169)

See all LEF1 products

View product citations for antibody GTX129186 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
IHC-P	Assay dependent

Not tested in other applications.

Calculated MW

44 kDa. ( Note )

Positive Control

Jurkat , Juarkat_NE

Product Note

Knockdown/Knockout validation was supported by references (PMID:35801925)

Predict Reactivity

Mouse, Bovine, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human LEF1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2885917

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

lymphoid enhancer binding factor 1 , LEF-1 , TCF10 , TCF1ALPHA , TCF7L3

Cellular Localization

Nucleus

Background

LEF1 is a nuclear protein that is expressed in pre-B and T cells. It binds to a functionally important site in the T-cell receptor-alpha (TCRA; MIM 186880) enhancer and confers maximal enhancer activity. LEF1 belongs to a family of regulatory proteins that share homology with high mobility group protein-1 (HMG1; MIM 163905) (Waterman et al., 1991 [PubMed 2010090]; van Genderen et al., 1994 [PubMed 7958926]).[supplied by OMIM]

Database

Gene ID: 51176 LEF1
UniProt: Q9UJU2 LEF1

Research Area

DATA IMAGES

GTX129186 WB Image

LEF1 antibody detects LEF1 protein by western blot analysis. Jurkat whole cell extracts and nuclear extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with LEF1 antibody (GTX129186) diluted by 1:1000.

GTX129186 IHC-P Image

LEF1 antibody detects LEF1 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded rat spleen.
LEF1 stained by LEF1 antibody (GTX129186) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min