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LIME antibody [LIME-06] (PE)

Anti-LIME antibody [LIME-06] (PE) used in Western Blot (WB). GTX80075

Cat. No. GTX80075

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 LIME-06

Isotype

 IgG1

Application

 WB, FACS

Reactivity

 Human
Package
100 μg ($399)
See all LIME products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
FACS Assay dependent
Not tested in other applications.

Calculated MW

31 kDa. ( Note )

Product Note

The antibody LIME-06 was raised against intracellular fragment corresponding to aa 141-295 of human LIME.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

15mM Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE. Protect from light.

Concentration

0.1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Bacterially expressed intracellular fragment corresponding to aa 141-295 of human LIME.

Purification

Purified by size-exclusion chromatography

Conjugation

Phycoerythrin (PE)

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

LIME , LIME1 , Lck interacting transmembrane adaptor 1 , dJ583P15.4

Cellular Localization

Cell membrane

Background

This gene encodes a transmembrane adaptor protein that links the T and B-cell receptor stimulation to downstream signaling pathways via its association with the Src family kinases Lck and Lyn, respectively. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Mar 2015]

Database

Research Area

DATA IMAGES

Anti-LIME antibody [LIME-06] (PE) used in Western Blot (WB). GTX80075

GTX80075 WB Image

Induction of LIME tyrosine phosphorylation.Peripheral blood T cells were left unstimulated (-) or stimulated with anti-human CD3 (MEM-92) or anti-human CD4 (MEM-16), and LIME was immunoprecipitated from laurylmaltoside lysates with the LIME-06 antibody (immunoaffinity sorbent) and analyzed by Western blotting to visualize tyrosine-phosphorylated LIME (top) and total LIME (bottom).

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399