APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
Assay dependent |
1:100-1:1000 |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Mouse
Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of mouse MAP2. The exact sequence is proprietary.
Purification
Affinity purified by Protein A.
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
microtubule-associated protein 2 , G1-397-34 , MAP-2 , Mtap-2 , Mtap2 , repro4
Cellular Localization
Cytoplasm, cytoskeleton,Cell projection, dendrite
Background
Enables microtubule binding activity. Involved in microtubule cytoskeleton organization; negative regulation of axon extension; and regulation of protein localization. Acts upstream of or within several processes, including establishment of cell polarity; microtubule bundle formation; and neuron projection development. Located in several cellular components, including axon; dendrite; and proximal neuron projection. Is expressed in several structures, including back skin; central nervous system; peripheral nervous system ganglion; retina; and trigeminal nerve. Orthologous to human MAP2 (microtubule associated protein 2). [provided by Alliance of Genome Resources, Apr 2022]
Database
Research Area
DATA IMAGES
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GTX637254 ICC/IF Image
MAP2 antibody [HL1656] detects MAP2 protein in dendrites, but not in axons, by immunofluorescent analysis.Sample: DIV9 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: MAP2 stained by MAP2 antibody [HL1656] (GTX637254) diluted at 1:250.Red: Tau, an axon marker, stained by Tau antibody [GT287] (GTX634809) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).
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GTX637254 IHC-P Image
MAP2 antibody [HL1656] detects MAP2 protein at cell membrane and cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded rat brain.MAP2 stained by MAP2 antibody [HL1656] (GTX637254) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX637254 IHC-P Image
MAP2 antibody [HL1656] detects MAP2 protein at cell membrane and cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse brain.MAP2 stained by MAP2 antibody [HL1656] (GTX637254) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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REFERENCE
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REVIEW
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