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MCP1 / CCL2 antibody [2D8]

Anti-MCP1 / CCL2 antibody [2D8] used in Western Blot (WB). GTX60582
Anti-MCP1 / CCL2 antibody [2D8] used in ELISA (ELISA). GTX60582
Anti-MCP1 / CCL2 antibody [2D8] used in Flow cytometry (FACS). GTX60582
Anti-MCP1 / CCL2 antibody [2D8] used in IHC (Paraffin sections) (IHC-P). GTX60582
Anti-MCP1 / CCL2 antibody [2D8] used in Western Blot (WB). GTX60582
Anti-MCP1 / CCL2 antibody [2D8] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60582
Anti-MCP1 / CCL2 antibody [2D8] used in IHC (Frozen sections) (IHC-Fr). GTX60582

Cat. No. GTX60582

Reference 7

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 2D8

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P, IHC-Fr, FACS, ELISA

Reactivity

 Human, Mouse, Rat, Monkey
Package
100 μg ($399)
See all MCP1 / CCL2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1/500 - 1/2000
ICC/IF 1/200 - 1/1000
IHC-P 1/200 - 1/1000
IHC-Fr Assay dependent
FACS 1/200 - 1/400
ELISA 1/10000
Not tested in other applications.

Calculated MW

11 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Purified recombinant fragment of human CCL2 expressed in E. Coli.

Purification

Protein G Purified

Conjugation

Unconjugated

RRID

AB_2887982

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

C-C motif chemokine ligand 2 , GDCF-2 , HC11 , HSMCR30 , MCAF , MCP-1 , MCP1 , SCYA2 , SMC-CF

Cellular Localization

Secreted

Background

This gene is one of several cytokine genes clustered on the q-arm of chromosome 17. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The protein encoded by this gene is structurally related to the CXC subfamily of cytokines. Members of this subfamily are characterized by two cysteines separated by a single amino acid. This cytokine displays chemotactic activity for monocytes and basophils but not for neutrophils or eosinophils. It has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis and atherosclerosis. It binds to chemokine receptors CCR2 and CCR4. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-MCP1 / CCL2 antibody [2D8] used in Western Blot (WB). GTX60582

GTX60582 WB Image

WB analysis of A549 (1), HeLa (2), Raw264.7 (3), L1210 (4), C6 (5), and COS-7 (6) using GTX60582 MCP1 / CCL2 antibody [2D8].

Anti-MCP1 / CCL2 antibody [2D8] used in ELISA (ELISA). GTX60582

GTX60582 ELISA Image

ELISA analysis of antigen using GTX60582 MCP1 / CCL2 antibody [2D8].
Black : Control antigen 100ng
Purple : Antigen 10ng
Blue : Antigen 50ng
Red : Antigen 100ng

Anti-MCP1 / CCL2 antibody [2D8] used in Flow cytometry (FACS). GTX60582

GTX60582 FACS Image

FACS analysis of A549 cells using GTX60582 MCP1 / CCL2 antibody [2D8].
Green : MCP1 / CCL2
Red : negative control

Anti-MCP1 / CCL2 antibody [2D8] used in IHC (Paraffin sections) (IHC-P). GTX60582

GTX60582 IHC-P Image

IHC-P analysis of human liver cancer tissue using GTX60582 MCP1 / CCL2 antibody [2D8].

Anti-MCP1 / CCL2 antibody [2D8] used in Western Blot (WB). GTX60582

GTX60582 WB Image

WB analysis of human CCL2 (AA: 1-99) recombinant protein using GTX60582 MCP1 / CCL2 antibody [2D8].

Anti-MCP1 / CCL2 antibody [2D8] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60582

GTX60582 ICC/IF Image

ICC/IF analysis of HepG2 cells using GTX60582 MCP1 / CCL2 antibody [2D8].
Green : MCP1 / CCL2
Blue: DRAQ5 fluorescent DNA dye
Red: Actin filaments

Anti-MCP1 / CCL2 antibody [2D8] used in IHC (Frozen sections) (IHC-Fr). GTX60582

GTX60582 IHC-Fr Image

The data was published in the journal J Neuroinflammation in 2014. PMID: 25012628

REFERENCE

Application Reference

TH Chang et al. Research Square 2023; The CCL2-CCR2 axis contributes to acquired osimertinib resistance in lung cancer by upregulating Zeb1 expression
Application : WB
Xu C et al. Oxid Med Cell Longev 2021; 2021 : 2502324 Chronic Intermittent Hypoxia Regulates CaMKII-Dependent MAPK Signaling to Promote the Initiation of Abdominal Aortic Aneurysm.
Application : WB
Reactivity : Mouse
Liao MF et al. Cells 2020; 9 (7) Granulocyte Colony Stimulating Factor (GCSF) Can Attenuate Neuropathic Pain by Suppressing Monocyte Chemoattractant Protein-1 (MCP-1) Expression, through Upregulating the Early MicroRNA-122 Expression in the Dorsal Root Ganglia.
Application : IHC-Fr
Reactivity : Rat
Guo SS et al. Cancer Lett 2019; 468 : 27-40 Endogenous production of C-C motif chemokine ligand 2 by nasopharyngeal carcinoma cells drives radioresistance-associated metastasis.
Application : WB
Reactivity : Human
Shang J et al. Toxicol Sci 2018; (Epub) Recovery from a myocardial infarction is impaired in male C57bl/6N mice acutely exposed to the bisphenols and phthalates that escape from medical devices used in cardiac surgery.
Application : WB
Reactivity : Mouse
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Application Reference
TH Chang et al. Research Square 2023; The CCL2-CCR2 axis contributes to acquired osimertinib resistance in lung cancer by upregulating Zeb1 expression
Application : WB
Xu C et al. Oxid Med Cell Longev 2021; 2021:2502324 Chronic Intermittent Hypoxia Regulates CaMKII-Dependent MAPK Signaling to Promote the Initiation of Abdominal Aortic Aneurysm.
Application : WB
Reactivity : Mouse
Liao MF et al. Cells 2020; 9 (7) Granulocyte Colony Stimulating Factor (GCSF) Can Attenuate Neuropathic Pain by Suppressing Monocyte Chemoattractant Protein-1 (MCP-1) Expression, through Upregulating the Early MicroRNA-122 Expression in the Dorsal Root Ganglia.
Application : IHC-Fr
Reactivity : Rat
Guo SS et al. Cancer Lett 2019; 468:27-40 Endogenous production of C-C motif chemokine ligand 2 by nasopharyngeal carcinoma cells drives radioresistance-associated metastasis.
Application : WB
Reactivity : Human
Shang J et al. Toxicol Sci 2018; (Epub) Recovery from a myocardial infarction is impaired in male C57bl/6N mice acutely exposed to the bisphenols and phthalates that escape from medical devices used in cardiac surgery.
Application : WB
Reactivity : Mouse
Wu H et al. Int J Mol Med 2018; (Epub) TIM-4 blockade of KCs combined with exogenous TGF-β injection helps to reverse acute rejection and prolong the survival rate of mice receiving liver allografts.
Application : WB
Reactivity : Mouse
McMillin M et al. J Neuroinflammation 2014; 11 (1):121 Neuronal CCL2 is upregulated during hepatic encephalopathy and contributes to microglia activation and neurological decline.
SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399