APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1/500 - 1/2000 |
| 1/200 - 1/1000 |
| 1/200 - 1/1000 |
| 1/200 - 1/400 |
| 1/10000 |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
Ascites
Preservative
0.03% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Antigen Species
Human
Immunogen
Purified recombinant fragment of human MAP2K7 expressed in E. Coli.
Purification
Unpurified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
mitogen-activated protein kinase kinase 7 , JNKK2 , MAPKK7 , MEK , MEK 7 , MKK7 , PRKMK7 , SAPKK-4 , SAPKK4
Cellular Localization
Nucleus,Cytoplasm
Background
The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase specifically activates MAPK8/JNK1 and MAPK9/JNK2, and this kinase itself is phosphorylated and activated by MAP kinase kinase kinases including MAP3K1/MEKK1, MAP3K2/MEKK2,MAP3K3/MEKK5, and MAP4K2/GCK. This kinase is involved in the signal transduction mediating the cell responses to proinflammatory cytokines, and environmental stresses. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found, but only one transcript variant has been supported and defined. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
![Anti-MEK7 antibody [4E5] used in ELISA (ELISA). GTX60522](/templates/default/js/lightbox/images/loading.gif "Anti-MEK7 antibody [4E5] used in ELISA (ELISA). GTX60522")
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GTX60522 ELISA Image
ELISA analysis of antigen using GTX60522 MEK7 antibody [4E5].
Black : Control antigen 100ng
Purple : Antigen 10ng
Blue : Antigen 50ng
Red : Antigen 100ng
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GTX60522 IHC-P Image
IHC-P analysis of human muscle tissue using GTX60522 MEK7 antibody [4E5].
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GTX60522 IHC-P Image
IHC-P analysis of lung cancer tissue using GTX60522 MEK7 antibody [4E5].
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GTX60522 FACS Image
FACS analysis of HeLa cells using GTX60522 MEK7 antibody [4E5].
Green : MEK7
Red : negative control
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GTX60522 ICC/IF Image
ICC/IF analysis of HeLa cells using GTX60522 MEK7 antibody [4E5].
Green : MEK7
Red: Actin filaments
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GTX60522 WB Image
WB analysis of human MEK7 (AA: 7-178) recombinant protein using GTX60522 MEK7 antibody [4E5].
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REFERENCE
There are currently no references for MEK7 antibody [4E5] (GTX60522). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for MEK7 antibody [4E5] (GTX60522). Be the first to share your experience with this product.
![Anti-MEK7 antibody [4E5] used in ELISA (ELISA). GTX60522](/upload/website/prouct_img/small/GTX60522/GTX60522_20170912_ELISA_w_23061123_236.webp "Anti-MEK7 antibody [4E5] used in ELISA (ELISA). GTX60522")
![Anti-MEK7 antibody [4E5] used in IHC (Paraffin sections) (IHC-P). GTX60522](/upload/website/prouct_img/small/GTX60522/GTX60522_20170912_IHC-P_1_w_23061123_779.webp "Anti-MEK7 antibody [4E5] used in IHC (Paraffin sections) (IHC-P). GTX60522")
![Anti-MEK7 antibody [4E5] used in IHC (Paraffin sections) (IHC-P). GTX60522](/upload/website/prouct_img/small/GTX60522/GTX60522_20170912_IHC-P_w_23061123_126.webp "Anti-MEK7 antibody [4E5] used in IHC (Paraffin sections) (IHC-P). GTX60522")
![Anti-MEK7 antibody [4E5] used in Flow cytometry (FACS). GTX60522](/upload/website/prouct_img/small/GTX60522/GTX60522_20170912_FACS_w_23061123_553.webp "Anti-MEK7 antibody [4E5] used in Flow cytometry (FACS). GTX60522")
![Anti-MEK7 antibody [4E5] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60522](/upload/website/prouct_img/small/GTX60522/GTX60522_20170912_ICCIF_w_23061123_205.webp "Anti-MEK7 antibody [4E5] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60522")
![Anti-MEK7 antibody [4E5] used in Western Blot (WB). GTX60522](/upload/website/prouct_img/small/GTX60522/GTX60522_20170912_WB_w_23061123_376.webp "Anti-MEK7 antibody [4E5] used in Western Blot (WB). GTX60522")
![Anti-MEK7 antibody [4E5] used in ELISA (ELISA). GTX60522](/upload/website/prouct_img/normal/GTX60522/GTX60522_20170912_ELISA_w_23061123_236.webp "Anti-MEK7 antibody [4E5] used in ELISA (ELISA). GTX60522")
![Anti-MEK7 antibody [4E5] used in IHC (Paraffin sections) (IHC-P). GTX60522](/upload/website/prouct_img/normal/GTX60522/GTX60522_20170912_IHC-P_1_w_23061123_779.webp "Anti-MEK7 antibody [4E5] used in IHC (Paraffin sections) (IHC-P). GTX60522")
![Anti-MEK7 antibody [4E5] used in IHC (Paraffin sections) (IHC-P). GTX60522](/upload/website/prouct_img/normal/GTX60522/GTX60522_20170912_IHC-P_w_23061123_126.webp "Anti-MEK7 antibody [4E5] used in IHC (Paraffin sections) (IHC-P). GTX60522")
![Anti-MEK7 antibody [4E5] used in Flow cytometry (FACS). GTX60522](/upload/website/prouct_img/normal/GTX60522/GTX60522_20170912_FACS_w_23061123_553.webp "Anti-MEK7 antibody [4E5] used in Flow cytometry (FACS). GTX60522")
![Anti-MEK7 antibody [4E5] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60522](/upload/website/prouct_img/normal/GTX60522/GTX60522_20170912_ICCIF_w_23061123_205.webp "Anti-MEK7 antibody [4E5] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60522")
![Anti-MEK7 antibody [4E5] used in Western Blot (WB). GTX60522](/upload/website/prouct_img/normal/GTX60522/GTX60522_20170912_WB_w_23061123_376.webp "Anti-MEK7 antibody [4E5] used in Western Blot (WB). GTX60522")