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MLK4 antibody [HL2234]

Recombinant antibody Orthogonal
Anti-MLK4 antibody [HL2234] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX638279
Anti-MLK4 antibody [HL2234] used in Western Blot (WB). GTX638279

Cat. No. GTX638279

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL2234

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all MLK4 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
Not tested in other applications.

Calculated MW

114 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human MLK4. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

mitogen-activated protein kinase kinase kinase 21 , MLK4 , dJ862P8.3

Cellular Localization

Cytoplasm

Background

Predicted to enable protein homodimerization activity and protein kinase activity. Predicted to be involved in protein autophosphorylation and signal transduction. Predicted to be active in cytoplasm. [provided by Alliance of Genome Resources, Apr 2022]

Database

Research Area

DATA IMAGES

Anti-MLK4 antibody [HL2234] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX638279

GTX638279 ICC/IF Image

MLK4 antibody [HL2234] detects MLK4 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: MLK4 stained by MLK4 antibody [HL2234] (GTX638279) diluted at 1:200.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-MLK4 antibody [HL2234] used in Western Blot (WB). GTX638279

GTX638279 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with MLK4 antibody [HL2234] (GTX638279) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations