APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:500-1:3000 |
Assay dependent |
Not tested in other applications.
Calculated MW
Product Note
This antibody is specific for human MX1 protein, and it does not cross react with human MX2 protein.
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant fragment of human MX1
Purification
Affinity purified by Protein A.
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
MX dynamin like GTPase 1 , IFI-78K , IFI78 , MX , MxA
Cellular Localization
Cytoplasm,Endoplasmic reticulum membrane,Cytoplasm, perinuclear region,Nucleus
Background
This gene encodes a guanosine triphosphate (GTP)-metabolizing protein that participates in the cellular antiviral response. The encoded protein is induced by type I and type II interferons and antagonizes the replication process of several different RNA and DNA viruses. There is a related gene located adjacent to this gene on chromosome 21, and there are multiple pseudogenes located in a cluster on chromosome 4. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2013]
Database
Research Area
DATA IMAGES
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GTX637955 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MX1 antibody [HL2051] (GTX637955) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX637955 WB Image
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MX1 antibody [HL2051] (GTX637955) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
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GTX637955 WB Image
Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with MX1 antibody [HL2051] (GTX637955) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX637955 IHC-P Image
MX1 antibody [HL2051] detects MX1 protein at cytoplasm and nucleus by immunohistochemical analysis.Sample: Paraffin-embedded cat kidney.MX1 stained by MX1 antibody [HL2051] (GTX637955) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX637955 IHC-P Image
MX1 antibody [HL2051] detects MX1 protein at cytoplasm and nucleus by immunohistochemical analysis.Sample: Paraffin-embedded human lung cancer.MX1 stained by MX1 antibody [HL2051] (GTX637955) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX637955 IHC-P Image
MX1 antibody [HL2051] detects MX1 protein at cytoplasm and nucleus by immunohistochemical analysis.Sample: Paraffin-embedded mouse kidney.MX1 stained by MX1 antibody [HL2051] (GTX637955) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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REFERENCE
There are currently no references for MX1 antibody [HL2051] (GTX637955). Be the first to share your publications with this product.
REVIEW
MX1 antibody [HL2051]
Cat. No. GTX637955
Rating
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( Average 5 based on 1 users reviews)
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Application
IHC (Paraffin sections)(IHC-P)
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( Average 5 based on 1 users reviews)
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Date :
Anonymous submitted on 09-Aug-2023
Application Tested :
IHC-P
Sample Species :
Ms
Sample :
mouse kidnay tissue
Permeabilization /Antigen retrieval :
EDTA pH 8.0, 0.5Hr
Primary Antibody :
1:100, 200, 400, room temp°C, 0.5Hr