GeneTex

  • Country / Location Selection

United States (US)

Muscleblind-like 1 antibody

Anti-Muscleblind-like 1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33335
Anti-Muscleblind-like 1 antibody used in IHC (Paraffin sections) (IHC-P). GTX33335
Anti-Muscleblind-like 1 antibody used in Western Blot (WB). GTX33335

Cat. No. GTX33335

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Rat
Package
100 μl ($399)
See all Muscleblind-like 1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:100
IHC-P 1:50 - 1:200
Not tested in other applications.

Calculated MW

42 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-382 of human MBNL1 (NP_066368.2).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

muscleblind like splicing regulator 1 , EXP , MBNL

Cellular Localization

Nucleus,Cytoplasm

Background

This gene encodes a member of the muscleblind protein family which was initially described in Drosophila melanogaster. The encoded protein is a C3H-type zinc finger protein that modulates alternative splicing of pre-mRNAs. Muscleblind proteins bind specifically to expanded dsCUG RNA but not to normal size CUG repeats and may thereby play a role in the pathophysiology of myotonic dystrophy. Mice lacking this gene exhibited muscle abnormalities and cataracts. Several alternatively spliced transcript variants have been described but the full-length natures of only some have been determined. The different isoforms are thought to have different binding specificities and/or splicing activities. [provided by RefSeq, Sep 2015]

Database

Research Area

DATA IMAGES

Anti-Muscleblind-like 1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33335

GTX33335 ICC/IF Image

ICC/IF analysis of MCF7 cells using GTX33335 Muscleblind-like 1 antibody.

Anti-Muscleblind-like 1 antibody used in IHC (Paraffin sections) (IHC-P). GTX33335

GTX33335 IHC-P Image

IHC-P analysis of rat heart tissue using GTX33335 Muscleblind-like 1 antibody.
Dilution : 1:100

Anti-Muscleblind-like 1 antibody used in Western Blot (WB). GTX33335

GTX33335 WB Image

WB analysis of various sample lysates using GTX33335 Muscleblind-like 1 antibody.
Dilution : 1:1000
Loading : 25μg per lane

REFERENCE

Application Reference

Lee KY et al. Hum Mol Genet 2022; 31 (18) : 3144-3160 Mice lacking MBNL1 and MBNL2 exhibit sudden cardiac death and molecular signatures recapitulating myotonic dystrophy.
Submit a Reference

REVIEW

There are currently no reviews for Muscleblind-like 1 antibody (GTX33335). Be the first to share your experience with this product.
Submit a Review
SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399