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NCAM antibody

Anti-NCAM antibody used in Flow cytometry (FACS). GTX04796
Anti-NCAM antibody used in Western Blot (WB). GTX04796
Anti-NCAM antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX04796
Anti-NCAM antibody used in IHC (Frozen sections) (IHC-Fr). GTX04796

Cat. No. GTX04796

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-Fr, FACS, LCI

Reactivity

 Human, Mouse, Rat
Package
50 μl ($669)
See all NCAM1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF Assay dependent
IHC-Fr Assay dependent
FACS Assay dependent
LCI Assay dependent
Not tested in other applications.

Calculated MW

95 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA

Preservative

0.05% Sodium Azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.85 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Rat

Immunogen

Peptide (C)DEKHIFSDDSSELTIR, corresponding to amino acid residues 261-276 of rat NCAM-1 (Accession P13596). Extracellular, N-terminus.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CD56 , MSK39 , NCAM , NCAM1 , neural cell adhesion molecule 1

Cellular Localization

Cell membrane

Background

cell adhesion molecule; involved in neuronal cell adhesion [RGD, Feb 2006]

Database

Research Area

DATA IMAGES

Anti-NCAM antibody used in Flow cytometry (FACS). GTX04796

GTX04796 FACS Image

FACS analysis of NK-92MI cells using GTX04796 NCAM antibody.
Green : Primary antibody
Red : Secondary antibody only
Black : Unlabeled cells
Dilution : 5 μg/ml

Anti-NCAM antibody used in Western Blot (WB). GTX04796

GTX04796 WB Image

WB analysis of various samples using GTX04796 NCAM antibody prior incubated with (Lane 6-10) or without (Lane 1-5) blocking peptide.
Lane 1 and 6 : Mouse brain tissue lysate
Lane 2 and 7 : Rat cerebellum tissue lysate
Lane 3 and 8 : Rat eye tissue lysate
Lane 4 and 9 : SH-SY5Y whole cell lysate
Lane 5 and 10 : PC12 whole cell lysate
Dilution :
Lane 1-3 & 6-8 : 1:400
Lane 4-5 & 9-10 : 1:200

Anti-NCAM antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX04796

GTX04796 ICC/IF Image

ICC/IF analysis of PC12 cells using GTX04796 NCAM antibody.
Extracellular staining of cells merged with a live view of the cells.
Dilution : 1:100

Anti-NCAM antibody used in IHC (Frozen sections) (IHC-Fr). GTX04796

GTX04796 IHC-Fr Image

IHC-Fr analysis of rat olfactory bulb tissue using GTX04796 NCAM antibody.
Green : Primary antibody
Blue : DAPI
NCAM staining appears in glomeruli (G) and is particularly intense in the outer border (arrows)
Dilution : 1:300

REFERENCE

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Package List Price ($)
$ 669