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NF-M antibody

Anti-NF-M antibody used in Western Blot (WB). GTX30441
Anti-NF-M antibody used in IHC (Frozen sections) (IHC-Fr). GTX30441
Anti-NF-M antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX30441

Cat. No. GTX30441

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Fr

Reactivity

 Human, Mouse, Rat, Bovine, Cat, Pig, Avian
Package
50 μl ($399)
See all NF-M products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:5000 - 1:10000
ICC/IF 1:500 - 1:1000
IHC-P 1:1000 - 1:5000
IHC-Fr 1:1000 - 1:5000
Not tested in other applications.

Calculated MW

96 kDa. ( Note )

Product Note

Specifically recognizes the evolutionarily conserved extreme C-terminal region of Neurofilament Medium (~145-170kDa).

PROPERTIES

Form

Liquid

Buffer

Serum

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Rat

Immunogen

Recombinant rat Neurofilament Medium fusion protein corresponding to the C-terminus [UniProt# P12839]

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

neurofilament medium , Nef3 , Nfm

Database

Research Area

DATA IMAGES

Anti-NF-M antibody used in Western Blot (WB). GTX30441

GTX30441 WB Image

WB analysis of rat cerebellum tissue using GTX30441 NF-M antibody.

Anti-NF-M antibody used in IHC (Frozen sections) (IHC-Fr). GTX30441

GTX30441 IHC-Fr Image

IHC-Fr analysis of rat cerebral cortex tissue using GTX30441 NF-M antibody. This immunostaining reveals the perikarya of pyramidal neurons and dendrites as well as axons surrounding the neurons.
Red : primary antibody
Green : beta-adrendergic receptor kinase 1
Dilution : 1:5000

Anti-NF-M antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX30441

GTX30441 ICC/IF Image

ICC/IF analysis of mixed neuron/glia cell cultures using GTX30441 NF-M antibody.
Red : NF-M antibody
Green : Fox1 antibody
Blue : DAPI

REFERENCE

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REVIEW

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Package List Price ($)
$ 399