APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:2000-1:10000 |
1:400 |
1:20000-1:100000 |
2 μL |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
Filtered-steriled serum
Preservative
0.01% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Antigen Species
Human
Immunogen
NF-Y (A subunit specific) peptide corresponding to a region near the N-terminus of the human protein conjugated to KLH.
Purification
Unpurified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
nuclear transcription factor Y subunit alpha , CBF-A , CBF-B , HAP2 , NF-YA
Cellular Localization
Nucleus
Background
Nuclear transcription factor Y subunit alpha is a protein that in humans is encoded by the NFYA gene. The protein encoded by this gene is one subunit of a trimeric complex, forming a highly conserved transcription factor that binds to CCAAT motifs in the promoter regions in a variety of genes. Subunit A associates with a tight dimer composed of the B and C subunits, resulting in a trimer that binds to DNA with high specificity and affinity. The sequence specific interactions of the complex are made by the A subunit, suggesting a role as the regulatory subunit. In addition, there is evidence of post-transcriptional regulation in this gene product, either by protein degradation or control of translation. Further regulation is represented by alternative splicing in the glutamine-rich activation domain, with clear tissue-specific preferences for the two isoforms. Anti-NF-Y is ideal for investigators involved in transcription factor research.
Database
Research Area
DATA IMAGES
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GTX48667 IHC-P Image
GeneTex's anti-NF-Y (A Subunit) antibody was diluted at 10 μg/mL for 1 h at RT to detect NF-YA in human prostate tissue. Tissue was formalin fixed paraffin embedded. Antigen retrieval: not required. Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. The image shows the localization of antibody as the precipitated red signal with a hematoxylin purple nuclear counterstain. Localization: NF-Y is nuclear.
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GTX48667 WB Image
CHO-7 cells were cultured in the absence (I) or presence (S) of cholesterol.
Load: 35 μg per lane. Primary antibody: NF-YA antibody at 1:1,000 for overnight at 4ºC. Secondary antibody: HRP conjugated Goat-anti-Rabbit IgG [H&L] secondary antibody at 1:10,000 for 45 min at RT. Predicted/Observed size: NF-YA recognized as a doublet of ~ 42 kDa in size. Other band(s): none.
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GTX48667 IHC-P Image
IHC-P analysis of human prostate tissue using GTX48667 NFYA antibody. Dilution : 10 μg/mL
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REFERENCE
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REVIEW
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