APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:400 |
Assay dependent |
Assay dependent |
Not tested in other applications.
Calculated MW
Positive Control
The peptide used to generate this antibody is available for purchase (GTX28006).
Product Note
This antibody reacts with NFkB p50 and p105.
PROPERTIES
Form
Liquid
Buffer
PBS, 0.2% Gelatin
Preservative
0.1% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.
Concentration
0.2 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Peptide mapping to the carboxy terminus of human NFkB p50.
Purification
Purified by affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
nuclear factor kappa B subunit 1 , CVID12 , EBP-1 , KBF1 , NF-kB1 , NF-kappa-B1 , NF-kappaB , NFKB-p105 , NFKB-p50 , NFkappaB , p105 , p50
Cellular Localization
Nucleus , Cytoplasm
Background
This gene encodes a 105 kD protein which can undergo cotranslational processing by the 26S proteasome to produce a 50 kD protein. The 105 kD protein is a Rel protein-specific transcription inhibitor and the 50 kD protein is a DNA binding subunit of the NF-kappa-B (NFKB) protein complex. NFKB is a transcription regulator that is activated by various intra- and extra-cellular stimuli such as cytokines, oxidant-free radicals, ultraviolet irradiation, and bacterial or viral products. Activated NFKB translocates into the nucleus and stimulates the expression of genes involved in a wide variety of biological functions. Inappropriate activation of NFKB has been associated with a number of inflammatory diseases while persistent inhibition of NFKB leads to inappropriate immune cell development or delayed cell growth. Alternative splicing results in multiple transcript variants encoding different isoforms, at least one of which is proteolytically processed. [provided by RefSeq, Feb 2016]
Database
Research Area
DATA IMAGES
|
GTX27971 WB Image
WB analysis of HeLa (A), HL-60 (B), and NIH/3T3 (C) whole cell lysates using GTX27971 NFkB p105/p50 antibody.
|
REFERENCE
There are currently no references for NFkB p105/p50 antibody (GTX27971). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for NFkB p105/p50 antibody (GTX27971). Be the first to share your experience with this product.