NFkB p65 (acetyl Lys310) antibody

Cat. No. GTX20308

Reference 1

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, IHC-P
Reactivity	Human, Mouse, Rat
Package	100 μl ($399)

See all NFkB p65 products

View product citations for antibody GTX20308 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:2000
IHC-P	1:100-1:300

Not tested in other applications.

Calculated MW

60 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 0.5% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthesized peptide derived from human NFkappaB-p65 around the acetylation site of K310 at 250-330 aa.

Purification

Purified by antigen-affinity chromatography
From serum

Conjugation

Unconjugated

RRID

AB_2887577

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

NFKB3,RELA,RELA protooncogene, NFkB subunit,p65,NFkB p65,NF-κb p65,RELA proto-oncogene, NF-kB subunit

Cellular Localization

Nucleus,Cytoplasm

Background

NF-kappa-B is a ubiquitous transcription factor involved in several biological processes. It is held in the cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor, NF-kappa-B moves to the nucleus and activates transcription of specific genes. NF-kappa-B is composed of NFKB1 or NFKB2 bound to either REL, RELA, or RELB. The most abundant form of NF-kappa-B is NFKB1 complexed with the product of this gene, RELA. Four transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]

Database

Gene ID: 5970 RELA
UniProt: Q04206 RELA

Research Area

DATA IMAGES

GTX20308 IHC-P Image

IHC-P analysis of rat spinal cord tissue using GTX20308 NFkB p65 (acetyl Lys310) antibody. Negative control (the lower left coner) was secondary antibody only.
Antigen retrieval : Sodium citrate pH6.0 was used for antibody retrieval (>98ºC, 20min)
Dilution : 1:200

GTX20308 IHC-P Image

IHC-P analysis of rat testis tissue using GTX20308 NFkB p65 (acetyl Lys310) antibody. Negative control (the lower left coner) was secondary antibody only.
Antigen retrieval : Sodium citrate pH6.0 was used for antibody retrieval (>98ºC, 20min)
Dilution : 1:200

GTX20308 IHC-P Image

IHC-P analysis of human stomach cancer tissue using GTX20308 NFkB p65 (acetyl Lys310) antibody. Negative control (the lower left coner) was secondary antibody only.
Antigen retrieval : Sodium citrate pH6.0 was used for antibody retrieval (>98ºC, 20min)
Dilution : 1:200

GTX20308 WB Image

WB analysis of HeLa cell treated with Doxorubicin using GTX20308 NFkB p65 (acetyl Lys310) antibody.

GTX20308 IHC-P Image

IHC-P analysis of mouse brain tissue using GTX20308 NFkB p65 (acetyl Lys310) antibody. Negative control (the lower left coner) was secondary antibody only.
Antigen retrieval : Sodium citrate pH6.0 was used for antibody retrieval (>98ºC, 20min)
Dilution : 1:200

GTX20308 IHC-P Image

IHC-P analysis of rat spleen tissue using GTX20308 NFkB p65 (acetyl Lys310) antibody. Negative control (the lower left coner) was secondary antibody only.
Antigen retrieval : Sodium citrate pH6.0 was used for antibody retrieval (>98ºC, 20min)
Dilution : 1:200

GTX20308 IHC-P Image

IHC-P analysis of mouse spleen tissue using GTX20308 NFkB p65 (acetyl Lys310) antibody. Negative control (the lower left coner) was secondary antibody only.
Antigen retrieval : Sodium citrate pH6.0 was used for antibody retrieval (>98ºC, 20min)
Dilution : 1:200

GTX20308 IHC-P Image

IHC-P analysis of human stomach tissue using GTX20308 NFkB p65 (acetyl Lys310) antibody. Negative control (the lower left coner) was secondary antibody only.
Antigen retrieval : Sodium citrate pH6.0 was used for antibody retrieval (>98ºC, 20min)
Dilution : 1:200

GTX20308 IHC-P Image

IHC-P analysis of rat lung tissue using GTX20308 NFkB p65 (acetyl Lys310) antibody. Negative control (the lower left coner) was secondary antibody only.
Antigen retrieval : Sodium citrate pH6.0 was used for antibody retrieval (>98ºC, 20min)
Dilution : 1:200

REFERENCE

Application Reference

Chen CY et al. Front Oncol 2020; 10 : 216 Hydroxygenkwanin Inhibits Class I HDAC Expression and Synergistically Enhances the Antitumor Activity of Sorafenib in Liver Cancer Cells.

Application : WB

Reactivity : Human

Submit a Reference

REVIEW

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Application Reference

Chen CY et al. Front Oncol 2020; 10:216 Hydroxygenkwanin Inhibits Class I HDAC Expression and Synergistically Enhances the Antitumor Activity of Sorafenib in Liver Cancer Cells.

Application : WB

Reactivity : Human

SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf

Package

List Price ($)

100 μl

$ 399

Qty

NFkB p65 (acetyl Lys310) antibody

Cat. No. GTX20308

Host

Clonality

Isotype

Application

Reactivity

APPLICATION

Application Note

Calculated MW

PROPERTIES

Form

Buffer

Preservative

Storage

Concentration

Antigen Species

Immunogen

Purification

Conjugation

RRID

Note

TARGET

Synonyms

Cellular Localization

Background

Database

Research Area

DATA IMAGES

GTX20308 IHC-P Image

GTX20308 IHC-P Image

GTX20308 IHC-P Image

GTX20308 WB Image

GTX20308 IHC-P Image

GTX20308 IHC-P Image

GTX20308 IHC-P Image

GTX20308 IHC-P Image

GTX20308 IHC-P Image

REFERENCE

Application Reference

REVIEW

Product

Research

Support

Company

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