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NFkB p65 antibody

Anti-NFkB p65 antibody used in Western Blot (WB). GTX27970
Anti-NFkB p65 antibody used in IHC (Paraffin sections) (IHC-P). GTX27970

Cat. No. GTX27970

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, IP, EMSA

Reactivity

 Human, Mouse, Rat
Package
1 ml ($399)
See all NFkB p65 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:400 - 1:1000
IHC-P Assay dependent
IP Assay dependent
EMSA Assay dependent
Not tested in other applications.

Calculated MW

60 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 0.2% Gelatin

Preservative

0.1% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide mapping to the carboxy terminus of human NFkB p65.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

RRID

AB_372499

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

RELA proto-oncogene, NF-kB subunit , CMCU , NFKB3 , p65

Cellular Localization

Nucleus , Cytoplasm

Background

NF-kappa-B is a ubiquitous transcription factor involved in several biological processes. It is held in the cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor, NF-kappa-B moves to the nucleus and activates transcription of specific genes. NF-kappa-B is composed of NFKB1 or NFKB2 bound to either REL, RELA, or RELB. The most abundant form of NF-kappa-B is NFKB1 complexed with the product of this gene, RELA. Four transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]

Database

Research Area

DATA IMAGES

Anti-NFkB p65 antibody used in Western Blot (WB). GTX27970

GTX27970 WB Image

WB analysis of A431 (A) and HeLa (B) whole cell lysates using GTX27970 NFkB p65 antibody.

Anti-NFkB p65 antibody used in IHC (Paraffin sections) (IHC-P). GTX27970

GTX27970 IHC-P Image

IHC-P analysis of human diffuse large B cell lymphoma using GTX27970 NFkB p65 antibody.

REFERENCE

Application Reference

Ekstrom M et al. Am J Physiol Endocrinol Metab 2010; 299 (2) : E234-40 Systemic inflammation activates the nuclear factor-kappaB regulatory pathway in adipose tissue.
Application : IHC-P
Reactivity : Human
Halle M et al. J Am Coll Cardiol 2010; 55 (12) : 1227-36 Sustained inflammation due to nuclear factor-kappa B activation in irradiated human arteries.
Application : IHC-P
Reactivity : Human
Merzianu M et al. Mod Pathol 2006; 19 (7) : 891-8 Nuclear BCL-10 expression is common in lymphoplasmacytic lymphoma/Waldenstr?m macroglobulinemia and does not correlate with p65 NF-kappaB activation.
Application : IHC-P
Reactivity : Human
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Package List Price ($)
$ 399